Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Bankowa, Katowice, Poland.
Department of Animal Taxonomy and Ecology, Faculty of Biology, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego, Poznań, Poland.
PLoS One. 2022 Mar 18;17(3):e0261485. doi: 10.1371/journal.pone.0261485. eCollection 2022.
Anhydrobiosis is considered to be an adaptation of important applicative implications because it enables resistance to the lack of water. The phenomenon is still not well understood at molecular level. Thus, a good model invertebrate species for the research is required. The best known anhydrobiotic invertebrates are tardigrades (Tardigrada), considered to be toughest animals in the world. Hypsibius. exemplaris is one of the best studied tardigrade species, with its name "exemplaris" referring to the widespread use of the species as a laboratory model for various types of research. However, available data suggest that anhydrobiotic capability of the species may be overestimated. Therefore, we determined anhydrobiosis survival by Hys. exemplaris specimens using three different anhydrobiosis protocols. We also checked ultrastructure of storage cells within formed dormant structures (tuns) that has not been studied yet for Hys. exemplaris. These cells are known to support energetic requirements of anhydrobiosis. The obtained results indicate that Hys. exemplaris appears not to be a good model species for anhydrobiosis research.
抗干燥被认为是一种具有重要应用意义的适应，因为它使生物能够抵抗缺水。然而，这种现象在分子水平上还没有被很好地理解。因此，需要一种好的无脊椎动物模型来进行研究。最著名的抗干燥无脊椎动物是缓步动物（Tardigrada），它们被认为是世界上最顽强的动物。水熊虫（Hypsibius. exemplaris）是研究最多的缓步动物物种之一，其名称“exemplaris”是指该物种被广泛用作各种类型研究的实验室模型。然而，现有数据表明，该物种的抗干燥能力可能被高估了。因此，我们使用三种不同的抗干燥方案来确定水熊虫（Hypsibius. exemplaris）的抗干燥生存能力。我们还检查了尚未研究过的形成休眠结构（tuns）内储存细胞的超微结构，这些细胞已知支持抗干燥的能量需求。所得结果表明，水熊虫（Hypsibius. exemplaris）似乎不是抗干燥研究的良好模型物种。
