Department of Biological Sciences, Virginia Tech, Blacksburg, VA,USA.
Department of Entomology, Virginia Tech, Blacksburg, VA,USA.
J Insect Sci. 2022 Mar 1;22(2). doi: 10.1093/jisesa/ieac016.
Laboratory experiments have advanced our understanding of honey bee (Apis mellifera) responses to environmental factors, but removal from the hive environment may also impact physiology. To examine whether the laboratory environment alters the honey bee gut bacterial community and immune responses, we compared bacterial community structure (based on amplicon sequence variant relative abundance), total bacterial abundance, and immune enzyme (phenoloxidase and glucose oxidase) activity of cohort honey bee workers kept under laboratory and hive conditions. Workers housed in the laboratory showed differences in the relative abundance of their core gut taxa, an increase in total gut bacterial abundance, and reduced phenoloxidase activity, compared to bees housed in hives.
实验室实验提高了我们对蜜蜂（Apis mellifera）对环境因素反应的理解，但从蜂巢环境中取出也可能影响生理机能。为了研究实验室环境是否改变了蜜蜂肠道细菌群落和免疫反应，我们比较了在实验室和蜂巢条件下饲养的工蜂的细菌群落结构（基于扩增子序列变异相对丰度）、总细菌丰度和免疫酶（酚氧化酶和葡萄糖氧化酶）活性。与在蜂巢中饲养的蜜蜂相比，在实验室中饲养的工蜂的核心肠道分类群的相对丰度、总肠道细菌丰度增加和酚氧化酶活性降低存在差异。
