“X”标记位置：从 CasX 中挖掘基因编辑的“金子”。
"X" marks the spot: Mining the gold in CasX for gene editing.
机构信息
Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA.
Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA; Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306, USA.
出版信息
Mol Cell. 2022 Mar 17;82(6):1083-1085. doi: 10.1016/j.molcel.2022.02.024.
Abstract
In this issue of Molecular Cell, Tsuchida et al. (2022) present a successful structure-guided effort in improving genome-editing efficiencies of CRISPR-CasX from Deltaproteobacteria (DpbCasX) and Planctomycetes (PlmCasX). Engineered variants that stabilize the active conformational state improved the catalytic efficiency by ∼10-20 fold in vitro and mean-editing efficiency by ∼2-3 fold in human cells.
摘要
在本期《分子细胞》中，Tsuchida 等人（2022 年）成功进行了一项基于结构的研究，改进了来自δ变形菌（DpbCasX）和浮霉菌门（PlmCasX）的 CRISPR-CasX 的基因组编辑效率。工程变体稳定了活性构象状态，使体外催化效率提高了约 10-20 倍，人类细胞中的平均编辑效率提高了约 2-3 倍。
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