Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA.
Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA; Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306, USA.
Mol Cell. 2022 Mar 17;82(6):1083-1085. doi: 10.1016/j.molcel.2022.02.024.
In this issue of Molecular Cell, Tsuchida et al. (2022) present a successful structure-guided effort in improving genome-editing efficiencies of CRISPR-CasX from Deltaproteobacteria (DpbCasX) and Planctomycetes (PlmCasX). Engineered variants that stabilize the active conformational state improved the catalytic efficiency by ∼10-20 fold in vitro and mean-editing efficiency by ∼2-3 fold in human cells.
在本期《分子细胞》中，Tsuchida 等人（2022 年）成功进行了一项基于结构的研究，改进了来自δ变形菌（DpbCasX）和浮霉菌门（PlmCasX）的 CRISPR-CasX 的基因组编辑效率。工程变体稳定了活性构象状态，使体外催化效率提高了约 10-20 倍，人类细胞中的平均编辑效率提高了约 2-3 倍。
