检测与清除：基于 CRISPR 的抗病毒技术。
Detect and destroy: CRISPR-based technologies for the response against viruses.
机构信息
Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA; Ph.D. Program in Virology, Harvard Medical School, Boston, MA 02115, USA.
Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA; Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA; Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA; Massachusetts Consortium on Pathogen Readiness, Boston, MA 02115, USA.
出版信息
Cell Host Microbe. 2021 May 12;29(5):689-703. doi: 10.1016/j.chom.2021.04.003. Epub 2021 Apr 28.
Despite numerous viral outbreaks in the last decade, including a devastating global pandemic, diagnostic and therapeutic technologies remain severely lacking. CRISPR-Cas systems have the potential to address these critical needs in the response against infectious disease. Initially discovered as the bacterial adaptive immune system, these systems provide a unique opportunity to create programmable, sequence-specific technologies for detection of viral nucleic acids and inhibition of viral replication. This review summarizes how CRISPR-Cas systems-in particular the recently discovered DNA-targeting Cas12 and RNA-targeting Cas13, both possessing a unique trans-cleavage activity-are being harnessed for viral diagnostics and therapies. We further highlight the numerous technologies whose development has accelerated in response to the COVID-19 pandemic.
尽管在过去十年中发生了许多病毒爆发，包括一场毁灭性的全球大流行，但诊断和治疗技术仍然严重缺乏。CRISPR-Cas 系统有可能满足应对传染病的这些关键需求。这些系统最初被发现是细菌适应性免疫系统，为检测病毒核酸和抑制病毒复制提供了一种独特的可编程、序列特异性技术。本综述总结了 CRISPR-Cas 系统，特别是最近发现的靶向 DNA 的 Cas12 和靶向 RNA 的 Cas13，它们都具有独特的转位切割活性，如何被用于病毒诊断和治疗。我们还进一步强调了许多技术的发展，这些技术的发展是为了应对 COVID-19 大流行而加速的。
Zotero 插件