Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.
Department of Veterinary Sciences, University of Messina, Messina, Italy.
Acta Histochem. 2022 Apr;124(3):151876. doi: 10.1016/j.acthis.2022.151876. Epub 2022 Mar 15.
Rodlet cells (RCs) have always been an enigma for scientists. RCs have been given a variety of activities over the years, including ion transport, osmoregulation, and sensory function. These cells, presumably as members of the granulocyte line, are present only in teleosts and play a role in the innate immune response. RCs are migratory cells found in a variety of organs, including skin, vascular, digestive, uropoietic, reproductive, and respiratory systems, and present distinct physical properties that make them easily recognizable in tissues and organs. The development of RCs can be divided into four stages: granular, transitional, mature, and ruptured, having different morphological characteristics. Our study aims to characterize the different stages of these cells by histomorphological and histochemical techniques. Furthermore, we characterized these cells at all stages with peroxidase and fluorescence immunohistochemical techniques using different antibodies: S100, tubulin, α-SMA, piscidin, and for the first time TLR-2. From our results, the immunoreactivity of these cells to the antibodies performed may confirm that RCs play a role in fish defense mechanisms, helping to expand the state of the art on immunology and immune cells of teleosts.
杆状细胞（RCs）一直是科学家们的一个谜。多年来，RCs 被赋予了各种活动，包括离子转运、渗透调节和感觉功能。这些细胞，推测作为粒细胞系的成员，仅存在于硬骨鱼中，在先天免疫反应中发挥作用。RCs 是迁移细胞，存在于多种器官中，包括皮肤、血管、消化、泌尿生殖、生殖和呼吸系统，并具有独特的物理特性，使它们在组织和器官中很容易识别。RCs 的发育可以分为四个阶段：颗粒状、过渡状、成熟状和破裂状，具有不同的形态特征。我们的研究旨在通过组织形态学和组织化学技术来描述这些细胞的不同阶段。此外，我们使用不同的抗体，通过过氧化物酶和荧光免疫组织化学技术对这些细胞进行了特征描述：S100、微管蛋白、α-SMA、鱼杀菌肽和 TLR-2，这是首次用于描述这些细胞。从我们的结果来看，这些细胞对抗体的免疫反应可能证实了 RCs 在鱼类防御机制中发挥作用，有助于扩展硬骨鱼免疫学和免疫细胞的现有知识。
