Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, USA.
Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, QC, Canada.
Vet Res. 2022 Mar 18;53(1):23. doi: 10.1186/s13567-022-01039-8.
Streptococcus suis is a significant economic and welfare concern in the swine industry. Pan-genome analysis provides an in-silico approach for the discovery of genes involved in pathogenesis in bacterial pathogens. In this study, we performed pan-genome analysis of 208 S. suis isolates classified into the pathogenic, possibly opportunistic, and commensal pathotypes to identify novel candidate virulence-associated genes (VAGs) of S. suis. Using chi-square tests and LASSO regression models, three accessory pan-genes corresponding to S. suis strain P1/7 markers SSU_RS09525, SSU_RS09155, and SSU_RS03100 (>95% identity) were identified as having a significant association with the pathogenic pathotype. The proposed novel SSU_RS09525 + /SSU_RS09155 + /SSU_RS03100 + genotype identified 96% of the pathogenic pathotype strains, suggesting a novel genotyping scheme for predicting the pathogenicity of S. suis isolates in North America. In addition, mobile genetic elements carrying antimicrobial resistance genes (ARGs) and VAGs were identified but did not appear to play a major role in the spread of ARGs and VAGs.
猪链球菌是养猪业中一个重大的经济和福利问题。泛基因组分析为发现细菌病原体发病机制相关基因提供了一种计算机方法。在这项研究中，我们对 208 株分类为致病性、可能机会性和共生性病原体的猪链球菌进行了泛基因组分析，以鉴定猪链球菌的新型候选毒力相关基因 (VAG)。使用卡方检验和 LASSO 回归模型，我们确定了三个与致病性病原型相关的辅助泛基因，它们对应于猪链球菌 P1/7 株的 SSU_RS09525、SSU_RS09155 和 SSU_RS03100 标记（>95%同一性）。提出的新型 SSU_RS09525+/SSU_RS09155+/SSU_RS03100+基因型鉴定了 96%的致病性病原型菌株，这表明了一种用于预测北美的猪链球菌分离株致病性的新型基因分型方案。此外，还鉴定了携带抗生素耐药基因 (ARGs) 和 VAG 的移动遗传元件，但它们似乎没有在 ARGs 和 VAG 的传播中起主要作用。
