Laboratório de Investigação em Microbiologia Médica, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; Laboratório de Microbiologia Clínica, Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal da Paraíba, João Pessoa, Brazil.
Laboratório de Investigação em Microbiologia Médica, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
J Glob Antimicrob Resist. 2022 Jun;29:207-211. doi: 10.1016/j.jgar.2022.03.007. Epub 2022 Mar 15.
Here we describe an IncQ1-like plasmid carrying bla in a new non-Tn4401 element found in Citrobacter werkmanii recovered from coastal water.
In vitro and in silico approaches were used to assess antimicrobial resistance determinants, as well as bla vicinities.
The LB-887 isolate showed a multidrug-resistant phenotype and was identified as C. werkmanii. Resistome analysis identified further acquired resistance determinants to β-lactams, aminoglycosides, sulphonamides/trimethoprim, tetracyclines, chloramphenicol, macrolides, rifampicin and fluoroquinolones. Plasmidome included incompatibility groups IncA, IncC2, IncR, Col and IncQ families. The bla was inserted on a new variant of NTE-II, called here NTE-IIe, carried by an InQ1-like plasmid of 7930 kb (pKPC-LB887). NTE-IIe differed from NTE-IId by the complete absence of ISKpn6-tnpA. The InQ1-like backbone harbouring this element had been described in Enterobacterales recovered from clinical and environmental settings.
Unravelling genetic structures related to bla dissemination in different settings may provide clues on the main forces driving evolution of this important resistance determinant. Indeed, the occurrence of bla in a new NTE variant from an environmental source highlights the ongoing evolution of this mobile genetic element. In addition, bla carriage on a small and highly mobilizable IncQ plasmid in C. freundii complex from recreational water, similar to others found in clinical isolates, may suggest its relevance for bla dissemination among different compartments.
本研究描述了一种携带 bla 的 IncQ1 样质粒，该质粒位于一种新的非 Tn4401 元件中，该元件存在于从沿海水中分离出的柠檬酸杆菌中。
采用体外和计算机模拟方法评估了抗生素耐药决定因子，以及 bla 周围区域。
LB-887 分离株表现出多药耐药表型，被鉴定为柠檬酸杆菌。耐药组分析确定了进一步获得的对β-内酰胺类、氨基糖苷类、磺胺类/甲氧苄啶、四环素类、氯霉素、大环内酯类、利福平、氟喹诺酮类的耐药决定因子。质粒组包括不相容群 IncA、IncC2、IncR、Col 和 IncQ 家族。bla 插入到一种新的 NTE-II 变体中，称为 NTE-IIe，该变体位于一个 7930kb 的 InQ1 样质粒（pKPC-LB887）上。NTE-IIe 与 NTE-IId 的不同之处在于完全缺失了 ISKpn6-tnpA。携带该元件的 InQ1 样骨架已在从临床和环境中分离出的肠杆菌科中被描述。
在不同环境中阐明与 bla 传播相关的遗传结构，可以提供有关推动这一重要耐药决定因子进化的主要力量的线索。事实上，bla 在一种来自环境源的新 NTE 变体中的出现突出了这种可移动遗传元件的持续进化。此外，在来自娱乐用水的弗氏柠檬酸杆菌复合体中，携带 bla 的小型且高度可移动的 IncQ 质粒与在临床分离株中发现的其他质粒相似，这可能表明其在不同环境中bla 传播的相关性。
