Webb L E, Johnson R C
Clin Biochem. 1986 Aug;19(4):212-5. doi: 10.1016/s0009-9120(86)80028-5.
We have developed a simple, specific, and sensitive method for plasma choline measurement based on the HPLC procedure of Potter et al. (J Neurochem 1983; 41: 188) for the measurement of acetylcholine and choline in neuronal tissue. The effluent from a reverse-phase column is mixed with choline oxidase in a post-column reaction coil to produce hydrogen peroxide which is monitored electrochemically. Plasma samples are prepared by deproteinization with perchloric acid. Choline is recovered quantitatively from the plasma, but an internal standard (homocholine) is added to compensate for any variation in electrode response. Choline can be measured in plasma samples containing less than 1 mumole per litre of plasma; the method response is linear in the 1-20 mumol/L range. Catecholamines and ascorbic acid do not interfere. The chromatography, enzymatic reactions, and electrochemistry all contribute to the specificity of the method.
我们基于Potter等人（《神经化学杂志》，1983年；41卷：188页）用于测定神经元组织中乙酰胆碱和胆碱的高效液相色谱法，开发了一种简单、特异且灵敏的血浆胆碱测定方法。反相柱的流出物在柱后反应盘管中与胆碱氧化酶混合，生成过氧化氢，通过电化学方法对其进行监测。血浆样品通过用高氯酸进行脱蛋白处理来制备。胆碱可从血浆中定量回收，但需添加内标物（高胆碱）以补偿电极响应的任何变化。胆碱可在每升血浆中胆碱含量低于1微摩尔的血浆样品中进行测定；该方法在1 - 20微摩尔/升范围内响应呈线性。儿茶酚胺和抗坏血酸不产生干扰。色谱法、酶促反应和电化学共同促成了该方法的特异性。
