Fossati T, Colombo M, Castiglioni C, Abbiati G
Istituto Ricerche LPB, Cinisello Balsamo (MI), Italy.
J Chromatogr B Biomed Appl. 1994 Jun 3;656(1):59-64. doi: 10.1016/0378-4347(94)00070-0.
A method for the determination of choline in human plasma is described, involving rapid purification of plasma samples and analysis by high-performance liquid chromatography using an on-column enzyme reactor with electrochemical detection. The linearity of the method was tested at choline levels from 3.5 to 28.6 microM in plasma. The recovery was 86% and was independent of the analyte concentration. The inter-assay precision (as coefficient of variation) and accuracy (as the deviation of the concentration found from the theoretical value) were always below 12% in the whole concentration range. The method was applied to the determination of plasma choline levels in eight healthy volunteers after intramuscular administration of L-alpha-glycerophosphorylcholine (1 g) or a placebo. Mean plasma choline levels in the placebo group ranged from 10.6 to 12.0 microM. After drug administration, the plasma choline level reached 35.1 microM in 30 min, then decreased gradually. Plasma choline levels became comparable in the treated and placebo groups 6-8 h after administration.
本文描述了一种测定人血浆中胆碱的方法，该方法包括血浆样品的快速纯化以及使用带有电化学检测的柱上酶反应器的高效液相色谱分析。该方法的线性在血浆中胆碱水平为3.5至28.6微摩尔时进行了测试。回收率为86%，且与分析物浓度无关。在整个浓度范围内，批间精密度（以变异系数表示）和准确度（以所测浓度与理论值的偏差表示）始终低于12%。该方法应用于八名健康志愿者肌肉注射L-α-甘油磷酸胆碱（1克）或安慰剂后血浆胆碱水平的测定。安慰剂组的平均血浆胆碱水平在10.6至12.0微摩尔之间。给药后，血浆胆碱水平在30分钟内达到35.1微摩尔，然后逐渐下降。给药6至8小时后，治疗组和安慰剂组的血浆胆碱水平变得相当。
