Damsma G, Flentge F
Department of Medicinal Chemistry, University of Groningen, The Netherlands.
J Chromatogr. 1988 Jun 24;428(1):1-8. doi: 10.1016/s0378-4347(00)83884-0.
An assay is described for the measurement of choline in plasma and red blood cells using liquid chromatography, an enzyme reactor and electrochemical detection after a simple sample pretreatment. The intra-assay coefficient of variation for choline was 6.2 and 3.8% in plasma and in red blood cells, respectively. Using this method we have re-investigated the presence of acetylcholine in blood constituents. We were not able to demonstrate acetylcholine with a limit of detection of 10 pmol per ml of plasma or per ml of red blood cells.
本文描述了一种在简单样品预处理后，使用液相色谱、酶反应器和电化学检测来测量血浆和红细胞中胆碱的分析方法。血浆和红细胞中胆碱的批内变异系数分别为6.2%和3.8%。使用该方法，我们重新研究了血液成分中乙酰胆碱的存在情况。我们未能检测出血浆或红细胞中每毫升10皮摩尔检测限的乙酰胆碱。
