Ingelfinger J R, Pratt R E, Ellison K E, Roth T P, Dzau V J
Clin Exp Hypertens A. 1986;8(4-5):687-94. doi: 10.3109/10641968609046586.
How local renin expression is regulated in many tissues has yet to be defined. In the present studies the ontogeny of renin in submandibular gland (SMG) and kidney of CD-1 mice was examined in order to determine whether renal and extrarenal renin are similarly expressed. In males, submandibular gland (SMG) renin and renin secretory rate increase at puberty as androgen rises. The ratio of secreted forms (1-chain renin cf. 2-chain) seen on Western blots shows predominance of 1-chain prior to puberty and 2-chain thereafter. This androgen influence on renin processing and secretion was supported by reversion to prepubertal patterns with castration of adult males and by conversion to male pattern in androgen treated females which otherwise have low renin levels. In contrast, renal renin remains unchanged throughout development. The influence of ontogeny on renal and SMG renin mRNA was examined by Northern analysis using renin cDNA: SMG renin mRNA increases from near zero to high levels at puberty while renal renin mRNA level is high throughout. Taken together, these data suggest that SMG renin is influenced by androgens, whereas renal renin is not. Apparent differences in tissue renin regulation may have important implications for local function of this enzyme.
许多组织中局部肾素表达是如何调控的尚未明确。在本研究中，检测了CD-1小鼠下颌下腺（SMG）和肾脏中肾素的个体发生情况，以确定肾内和肾外肾素的表达是否相似。在雄性小鼠中，随着雄激素水平在青春期升高，下颌下腺（SMG）肾素和肾素分泌率增加。蛋白质印迹上可见的分泌形式（1链肾素与2链肾素相比）的比例显示，青春期前1链占优势，之后2链占优势。成年雄性小鼠去势后恢复到青春期前模式，以及用雄激素处理原本肾素水平较低的雌性小鼠使其转变为雄性模式，均支持了雄激素对肾素加工和分泌的这种影响。相比之下，肾脏肾素在整个发育过程中保持不变。使用肾素cDNA通过Northern印迹分析检测了个体发生对肾脏和SMG肾素mRNA的影响：SMG肾素mRNA在青春期从接近零增加到高水平，而肾脏肾素mRNA水平在整个过程中都很高。综上所述，这些数据表明SMG肾素受雄激素影响，而肾脏肾素不受影响。组织肾素调控的明显差异可能对该酶的局部功能具有重要意义。
