Key Laboratory of Urban Environment and Health, Fujian Key Laboratory of Watershed Ecology, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Key Laboratory of Urban Environment and Health, Fujian Key Laboratory of Watershed Ecology, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China.
Environ Pollut. 2022 Jun 15;303:119176. doi: 10.1016/j.envpol.2022.119176. Epub 2022 Mar 16.
A better understanding of the spatiotemporal dynamics and influencing factors of sulfonamide antibiotic resistance genes (ARGs) distribution in subsurface flow constructed wetlands is essential to improve the ARGs removal efficiency. The spatiotemporal dynamics of sulfonamide ARGs were explored in the vertical upflow subsurface flow constructed wetland (VUSFCW). The results showed that the absolute abundance of ARGs presented a trend of bottom layer > middle layer > top layer. The relative abundance of ARGs decreased significantly from the bottom layer to the middle layer, but increased in the top layer. The bottom layer was the main stage to remove ARGs. The absolute abundance of ARGs at each point in summer was significantly higher than that in winter. Based on the spatiotemporal distribution of ARGs, the internal mechanism of ARGs dynamic change was explored by the partial least square path analysis model. The results showed that physical-chemical factors, microorganisms and antibiotics indirectly affected the spatiotemporal distribution of ARGs mainly through mobile genetic elements. The indirect influence coefficients of physical-chemical factors, microorganisms and antibiotics on the spatiotemporal distribution of ARGs were 0.505, 0.221 and 0.98 respectively. The direct influence coefficient of MGEs on the spatiotemporal distribution of ARGs was 0.895. The results of network analysis showed that the potential host species of ARGs in summer were more abundant than those in winter. The selection mode of sulfonamide ARGs to potential hosts was nonspecific. There is a risk of sulfonamide ARGs infecting pathogens in VUSFCW. Fortunately, VUSFCW has proven effective in reducing the absolute abundance of ARGs and the potential risk of pathogens carrying ARGs. These findings provide a model simulation and theoretical basis for effectively reducing the threat of ARGs.
更好地了解磺胺类抗生素抗性基因 (ARGs) 在地下流人工湿地中的时空动态及其影响因素，对于提高 ARGs 去除效率至关重要。本研究在垂直上向地下流人工湿地 (VUSFCW) 中探索了磺胺类 ARGs 的时空动态。结果表明，ARGs 的绝对丰度呈现底层>中层>顶层的趋势。从底层到中层，ARGs 的相对丰度显著降低，但在顶层增加。底层是去除 ARGs 的主要阶段。夏季每个点的 ARGs 绝对丰度明显高于冬季。基于 ARGs 的时空分布，通过偏最小二乘路径分析模型探讨了 ARGs 动态变化的内在机制。结果表明，物理化学因素、微生物和抗生素主要通过移动遗传元件间接影响 ARGs 的时空分布。物理化学因素、微生物和抗生素对 ARGs 时空分布的间接影响系数分别为 0.505、0.221 和 0.98。MGEs 对 ARGs 时空分布的直接影响系数为 0.895。网络分析的结果表明，夏季 ARGs 的潜在宿主种类比冬季更丰富。磺胺类 ARGs 对潜在宿主的选择模式是非特异性的。VUSFCW 中存在磺胺类 ARGs 感染病原体的风险。幸运的是，VUSFCW 已被证明能有效降低 ARGs 的绝对丰度和携带 ARGs 的病原体的潜在风险。这些发现为有效降低 ARGs 威胁提供了模型模拟和理论基础。
