School of Energy and Environment, Southeast University, Nanjing, 210096, PR China.
School of Environment, Nanjing Normal University, Jiangsu Centre for Collaborative Innovation in Geographical Information Resource Development and Application, Jiangsu Engineering Lab of Water and Soil Eco-remediation, Nanjing, 210023, PR China.
Chemosphere. 2020 Jun;248:126014. doi: 10.1016/j.chemosphere.2020.126014. Epub 2020 Jan 23.
Microbial fuel cell constructed wetlands (CW-MFCs) with different circuit operation conditions and hydraulic retention time (HRT) were constructed to evaluate their ability to remove and accumulate pharmaceutical and personal care products (PPCPs) (sulfadiazine (SDZ), carbamazepine (CBZ), naproxen (NPX) and ibuprofen (IBP)) during four months running process. The abundance level of corresponding sulfonamide antibiotic resistance genes (ARGs) was also investigated. The results showed that closed circuit operation of CW-MFC contributed to the decrease in mass loading of COD, NH-N, PPCPs, and wastewater toxicity in the effluent. Additionally, closed circuit operation with low HRT contributed to enhancing selected PPCP mass accumulation on electrodes by electro-adsorption, and thus the higher sulfonamide ARG abundance was detected in the electrodes and effluent. Moreover, the composition of bacteria was greatly influenced by the mass accumulation of PPCPs revealed by redundancy analysis results. Procrustes analysis results further demonstrated that bacterial community contributed greatly to the ARGs profiles. Therefore, ARGs with their host bacteria revealed by network analysis were partially deposited on electrode substrates, and thus ARGs were effectively accumulated on electrodes. Function analysis of the bacterial community from PICRUSt predicted metagenomes revealed that closed circuit mode enhanced the abundances of the function genes of metabolic and the multiple ARGs, suggesting that closed circuit operation exhibited positive effects on metabolic process and ARG accumulation in CW-MFC system.
构建了具有不同电路运行条件和水力停留时间（HRT）的微生物燃料电池湿地（CW-MFC），以评估它们在四个月的运行过程中去除和积累药物和个人护理产品（PPCPs）（磺胺嘧啶（SDZ）、卡马西平（CBZ）、萘普生（NPX）和布洛芬（IBP））的能力。还研究了相应的磺胺类抗生素抗性基因（ARGs）的丰度水平。结果表明，CW-MFC 的闭路运行有助于降低 COD、NH-N、PPCPs 和废水毒性的质量负荷。此外，闭路运行与低 HRT 有助于通过电吸附增强电极上选定 PPCP 的质量积累，因此在电极和流出物中检测到更高的磺胺类 ARG 丰度。此外，冗余分析结果表明，细菌的组成受到 PPCPs 质量积累的极大影响。Procrustes 分析结果进一步表明，细菌群落对 ARGs 图谱有很大贡献。因此，通过网络分析揭示的带有宿主细菌的 ARGs 部分沉积在电极基材上，从而有效地在电极上积累了 ARGs。从 PICRUSt 预测宏基因组中对细菌群落的功能分析表明，闭路模式增强了代谢和多个 ARGs 的功能基因的丰度，表明闭路操作对 CW-MFC 系统中的代谢过程和 ARG 积累具有积极影响。
