Brandon Bioscience, Tralee, Co. Kerry, Ireland.
Plant Biostimulant Group, Shannon Applied Biotechnology Centre, Munster Technological University-Kerry (South Campus), Clash, Tralee, Co. Kerry, Ireland; Brandon Bioscience, Tralee, Co. Kerry, Ireland.
Plant Physiol Biochem. 2022 May 15;179:44-57. doi: 10.1016/j.plaphy.2022.03.006. Epub 2022 Mar 12.
Reduction in the greenhouse gas (GHG) emissions and nitrogen (N) pollution of ground water by improving nitrogen use efficiency (NUE) in crops has become an intensively investigated research topic in pursuit of a more sustainable future. Although, distinct solutions have been proposed there are only a few reports documenting the detailed interplay between observed plant growth dynamics and changes in plant N related transcriptional and biochemical changes. It was previously demonstrated that the application of a formulated biostimulant (PSI-362) derived from Ascophyllum nodosum (ANE) improves N uptake in Arabidopsis thaliana and in barley. In this study, the effect of PSI-362 on the growth dynamics of wheat seedlings was evaluated at different biostimulant and N supplementation rates. Wheat grown on N deficient MS medium was also analysed from the first hour of the treatment until the depletion of the nutrients in the medium 9 days later. During this time the biomass increase measured for PSI-362 treated plants versus untreated controls was associated with increased nitrate uptake, with surplus N assimilated by the biomass in the form of glutamate, glutamine, free amino acids, soluble proteins, and chlorophyll. Phenotypical and biochemical analysis were supported by evaluation of differential expression of genetic markers involved in nitrate perception and transport (TaNRT1.1/NPF6.3), nitrate and nitrite reduction (TaNR1 and TaNiR1) and assimilation (TaGDH2, TaGoGAT, TaGS1). Finally, a comparative analysis of the precision biostimulant PSI-362 and two generic ANEs demonstrated that the NUE effect greatly differs depending on the ANE formulation used.
提高作物氮利用效率（NUE）以减少温室气体（GHG）排放和地下水氮污染已成为追求更可持续未来的研究热点。虽然已经提出了明确的解决方案，但只有少数报道记录了观察到的植物生长动态与植物 N 相关转录和生化变化之间的详细相互作用。先前的研究表明，应用一种源自泡叶藻（ANE）的配方生物刺激素（PSI-362）可提高拟南芥和大麦的氮吸收。在这项研究中，以不同生物刺激素和氮补充率评估了 PSI-362 对小麦幼苗生长动态的影响。在缺乏氮的 MS 培养基上生长的小麦也从处理的第一小时开始分析，直到 9 天后培养基中的养分耗尽。在此期间，与未处理对照相比，PSI-362 处理植物的生物量增加与硝酸盐吸收增加相关，过量的 N 以谷氨酸、谷氨酰胺、游离氨基酸、可溶性蛋白质和叶绿素的形式被生物量同化。表型和生化分析得到了参与硝酸盐感知和运输（TaNRT1.1/NPF6.3）、硝酸盐和亚硝酸盐还原（TaNR1 和 TaNiR1）和同化（TaGDH2、TaGoGAT、TaGS1）的遗传标记差异表达评估的支持。最后，对精确生物刺激素 PSI-362 和两种通用 ANE 的比较分析表明，NUE 效应取决于所用 ANE 配方的差异。
