Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai, 201210, P. R. China.
School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, P. R. China.
Adv Mater. 2022 May;34(19):e2201411. doi: 10.1002/adma.202201411. Epub 2022 Apr 3.
The development of minimally invasive cardiac patches, either as hemostatic dressing or treating myocardial infarction, is of clinical significance but remains a major challenge. Designing such patches often requires simultaneous consideration of several material attributes, including bioabsorption, non-toxicity, matching the mechanic properties of heart tissues, and working efficiently in wet and dynamic environments. Using genetically engineered multi-domain proteins, a printed bi-layer proteinaceous hydrogel patch for heart failure treatments is reported. The intrinsic self-healing nature of hydrogel materials physically enables seamless interfacial integration of two disparate hydrogel layers and functionally endows the cardiac patches with the combinatorial advantages of each layer. Leveraging the biocompatibility, structural stability, and tunable drug release properties of the bi-layer hydrogel, promising effects of hemostasis, fibrosis reduction, and heart function recovery on mice is demonstrated with two myocardium damage models. Moreover, this proteinaceous patch is proved biodegradable in vivo without any additive inflammations. In conclusion, this work introduces a promising new type of minimally invasive patch based on genetically modified double-layer protein gel for treating heart-related injuries or diseases.
微创心脏补片的开发，无论是用作止血敷料还是治疗心肌梗死，都具有重要的临床意义，但仍然是一个主要挑战。设计此类补片通常需要同时考虑几个材料属性，包括生物吸收性、无毒性、与心脏组织的力学性能相匹配，以及在潮湿和动态环境中高效工作。本研究报告了一种用于心力衰竭治疗的基于基因工程多结构域蛋白的双层蛋白水凝胶贴片。水凝胶材料的固有自修复特性在物理上实现了两个不同水凝胶层的无缝界面集成，并且在功能上赋予了心脏补片的每个层的组合优势。利用双层水凝胶的生物相容性、结构稳定性和可调节药物释放特性，通过两种心肌损伤模型，在小鼠中证明了止血、减少纤维化和恢复心脏功能的有希望的效果。此外，该蛋白贴片在体内被证明是可生物降解的，没有任何添加剂炎症。总之，本工作介绍了一种基于基因修饰双层蛋白凝胶的微创新型贴片，用于治疗与心脏相关的损伤或疾病。
