Wellawa Dinesh H, Lam Po-King S, White Aaron P, Gomis Susantha, Allan Brenda, Köster Wolfgang
Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada.
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
Front Microbiol. 2022 Mar 3;13:824052. doi: 10.3389/fmicb.2022.824052. eCollection 2022.
The roles of TonB mediated Fe (ferric iron) uptake via enterobactin (involving biosynthesis genes ) and Fe (ferrous iron) uptake through the FeoABC transporter are poorly defined in the context of chicken- interactions. Both uptake systems are believed to be the major contributors of iron supply in the life cycle. Current evidence suggests that these iron uptake systems play a major role in pathogenesis in mammals and as such, they represent promising antibacterial targets with therapeutic potential. We investigated the role of these iron uptake mechanisms regarding the ability of Enteritidis (SEn) strains to colonize in a chicken infection model. Further we constructed a bioluminescent reporter to sense iron limitation during gastrointestinal colonization of in chicken via imaging. Our data indicated that there is some redundancy between the ferric and ferrous iron uptake mechanisms regarding iron acquisition during SEn pathogenesis in chicken. We believe that this redundancy of iron acquisition in the host reservoir may be the consequence of adaptation to unique avian environments, and thus warrants further investigation. To our knowledge, this the first report providing direct evidence that both enterobactin synthesis and FeoABC mediated iron uptake contribute to the virulence of SEn in chickens.
在鸡的相互作用背景下，TonB介导的通过肠杆菌素摄取铁（铁离子，涉及生物合成基因）以及通过FeoABC转运蛋白摄取亚铁离子的作用尚不明确。这两种摄取系统都被认为是生命周期中铁供应的主要贡献者。目前的证据表明，这些铁摄取系统在哺乳动物发病机制中起主要作用，因此，它们是具有治疗潜力的有前景的抗菌靶点。我们研究了这些铁摄取机制在肠炎沙门氏菌（SEn）菌株在鸡感染模型中定殖能力方面的作用。此外，我们构建了一个生物发光报告基因，通过成像来检测鸡胃肠道定殖过程中的铁限制情况。我们的数据表明，在鸡SEn发病过程中铁摄取方面，铁离子和亚铁离子摄取机制之间存在一些冗余。我们认为，宿主储存库中铁摄取的这种冗余可能是适应独特禽类环境的结果，因此值得进一步研究。据我们所知，这是第一份提供直接证据表明肠杆菌素合成和FeoABC介导的铁摄取都有助于SEn在鸡体内毒力的报告。
