Beck T, Pollow K, Heubner A
Geburtshilfe Frauenheilkd. 1986 Aug;46(8):490-4. doi: 10.1055/s-2008-1036243.
The article reports on first experiences with immunohistochemical staining methods (ER-ICA and ER-D5) using monoclonal antibodies for determination of oestrogen receptor status in tissue slides. 95 primary breast cancers were analysed by ER-ICA; in addition, a subgroup of 31 cases was stained for ER-D5. The results of the two immunohistochemical procedures revealed a large measure of agreement with the standard ligand binding assay. The evaluation of the immunohistochemical staining reaction led to the formation of a staining Score: Score points of ER-ICA, not those of ER-D5, revealed good correlation with oestrogen receptor concentrations in cytosol, determined by standard ligand binding assay. The used staining Score was examined for intra- and inter-individual differences of evaluation and proved to be well reproducible. Further clinical studies have to prove how far immunohistochemical features can convey clinically relevant information not yielded by the steroid binding assay.
本文报道了使用单克隆抗体的免疫组织化学染色方法（ER-ICA和ER-D5）在组织切片中测定雌激素受体状态的首次经验。通过ER-ICA分析了95例原发性乳腺癌；此外，对31例病例的亚组进行了ER-D5染色。两种免疫组织化学方法的结果显示与标准配体结合试验有很大程度的一致性。免疫组织化学染色反应的评估导致了染色评分的形成：ER-ICA的评分点，而非ER-D5的评分点，与通过标准配体结合试验测定的细胞溶质中雌激素受体浓度显示出良好的相关性。对所使用的染色评分进行了个体内和个体间评估差异的检查，结果证明其具有良好的可重复性。进一步的临床研究必须证明免疫组织化学特征在多大程度上能够传达类固醇结合试验未提供的临床相关信息。
