钙调蛋白激酶激酶 2 通过 PI3K/PDK1/Akt 轴促进卵巢癌的进展。
CaMKK2 Promotes the Progression of Ovarian Carcinoma through the PI3K/PDK1/Akt Axis.
机构信息
Taizhou Hospital of Zhejiang Province affiliated to Wenzhou Medical University, Luqiao, Zhejiang 318050, China.
Wuhan Economic Development Zone (Hannan) People's Hospital, The department of obstetrics and gynecology, Wuhan, Hubei 430090, China.
出版信息
Comput Math Methods Med. 2022 Mar 11;2022:7187940. doi: 10.1155/2022/7187940. eCollection 2022.
OBJECTIVE
To explore the functional role of Calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2) in the progression of ovarian carcinoma (OC).
METHODS
RT-qPCR analysis and western blot were conducted to detect the mRNA and protein expression of CaMKK2, PI3K, PDK1 and Akt in OC tissues and cells, respectively. CCK-8 assay, transwell migration assay and flow cytometry were used to measure cell proliferation, migration and apoptosis, respectively.
RESULTS
CaMKK2, PI3K, PDK1 and Akt were highly expressed in OC tissues compared with the corresponding controls. CaMKK2 knockdown significantly suppressed the mRNA and protein expression of PI3K, PDK1 and Akt in HO8910 and OV90 cells. Moreover, CaMKK2 knockdown could dramatically repress cell proliferation, migration, and markedly elevate cell apoptosis in HO8910 and OV90 cells.
CONCLUSIONS
CaMKK2 played a promotion role in OC progression via activating the PI3K/PDK1/Akt axis.
目的
探讨钙/钙调蛋白依赖性蛋白激酶激酶 2（CaMKK2）在卵巢癌（OC）进展中的功能作用。
方法
分别通过 RT-qPCR 分析和 Western blot 检测 OC 组织和细胞中 CaMKK2、PI3K、PDK1 和 Akt 的 mRNA 和蛋白表达。CCK-8 assay、transwell 迁移 assay 和流式细胞术分别用于测量细胞增殖、迁移和凋亡。
结果
与相应对照相比，CaMKK2、PI3K、PDK1 和 Akt 在 OC 组织中高表达。CaMKK2 敲低显著抑制 HO8910 和 OV90 细胞中 PI3K、PDK1 和 Akt 的 mRNA 和蛋白表达。此外，CaMKK2 敲低可显著抑制 HO8910 和 OV90 细胞的增殖、迁移，并显著增加细胞凋亡。
结论
CaMKK2 通过激活 PI3K/PDK1/Akt 轴在 OC 进展中发挥促进作用。
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