Chen Dalei, Wu Zhijian, Wu Lu-Ning, Jiang Jingtao, Hu Gui-Nv
Department of Thyroid and Breast Surgery, Affiliated Dongyang Hospital of Wenzhou Medical University, Dongyang, China.
Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
Front Bioeng Biotechnol. 2022 Mar 2;10:830574. doi: 10.3389/fbioe.2022.830574. eCollection 2022.
The treatment of wounds remains a clinical challenge because of poor angiogenesis under the wound bed, and increasingly, the patients' need for functional and aesthetically pleasing scars. Previous reports have shown that Theaflavin can induce angiogenesis and terminate the progression of ischemic cardiovascular disease, but limited therapy is available for the management of cutaneous wounds. In this study, our work discovered that human umbilical vein endothelial cells (HUVECs) exposed to Theaflavin can alleviate apoptosis and cell dysfunction induced by tert-butyl hydroperoxide (TBHP). The cellular activity of HUVECs were assessed by cell tube formation, migration and adhesion. Mechanistically, Theaflavin protected HUVECs from TBHP-stimulated cell apoptosis through the activation of the phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT)/nuclear factor (erythroid-derived 2)-like 2 (Nrf2) axis, so Nrf2 silencing can partly eliminate the cytoprotective effect of Theaflavin treatment. In experiments, administering Theaflavin orally can enhance vascularization in regenerated tissues and accelerate wound healing. In summary, our data served as a novel evidence for the wound healing treatment with Theaflavin, and certified the potential mechanism of Theaflavin, which can be used as a potential agent for cutaneous wound therapy.
由于伤口床血管生成不良，以及患者对功能性和美观性瘢痕的需求日益增加，伤口治疗仍然是一项临床挑战。先前的报告表明，茶黄素可诱导血管生成并终止缺血性心血管疾病的进展，但用于治疗皮肤伤口的疗法有限。在本研究中，我们发现暴露于茶黄素的人脐静脉内皮细胞（HUVECs）可减轻叔丁基过氧化氢（TBHP）诱导的细胞凋亡和细胞功能障碍。通过细胞管形成、迁移和黏附来评估HUVECs的细胞活性。从机制上讲，茶黄素通过激活磷脂酰肌醇-3-激酶（PI3K）/蛋白激酶B（AKT）/核因子（红系衍生2）样2（Nrf2）轴保护HUVECs免受TBHP刺激的细胞凋亡，因此Nrf2沉默可部分消除茶黄素治疗的细胞保护作用。在实验中，口服茶黄素可增强再生组织中的血管生成并加速伤口愈合。总之，我们的数据为茶黄素用于伤口愈合治疗提供了新的证据，并证实了茶黄素的潜在机制，其可作为皮肤伤口治疗的潜在药物。
