Isaka Kazuichi, Sugawara Daichi, Yamazaki Hiroshi, Kimura Yuya, Osaka Toshifumi, Tsuneda Satoshi
Department of Applied Chemistry, Toyo University, Saitama, Japan.
Department of Civil and Environmental Engineering, Toyo University, Saitama, Japan.
Front Bioeng Biotechnol. 2022 Mar 4;10:851617. doi: 10.3389/fbioe.2022.851617. eCollection 2022.
Anaerobic ammonia oxidation (anammox) bacteria are inhibited by heavy metals at high concentrations but require trace amounts of some heavy-metal elements for growth and activity maintenance. The present study evaluates the long-term limitation effects of Se(VI), Zn(II), and Ni(II) on the start-up period of an anammox reactor. To strictly limit the levels of heavy metals in the reactor, all tests used ultrapure water as the influent synthetic wastewater and all reactors were installed in a clean booth. The anammox biomass was maintained through the gel entrapment technique. In the absence of Se(VI) and Ni(II), the anammox reactor start-up was 18.9 kg-N (m-carrier d) (nitrogen conversion rate (NCR) per gel carriers), indicating that Se(VI) and Ni(II) are not required or need not be continuously added to maintain the anammox process. Under Zn(II) limitation, the anammox process failed to start-up and the NCR tended to decrease rapidly. After readdition of 0.005 mg L of Zn(II), the NCR did not decline further and instead partially recovered at a very slow rate. The NCR was completely recovered after adding 0.020 mg L of Zn(II). These results reveal that Zn(II) limitation seriously affects the start-up of the anammox process while Se(VI) and Ni(II) are not required or need not be continuously added to the anammox process.
厌氧氨氧化（anammox）细菌在高浓度重金属环境下会受到抑制，但生长和维持活性需要微量的某些重金属元素。本研究评估了硒（VI）、锌（II）和镍（II）对厌氧氨氧化反应器启动阶段的长期限制影响。为严格限制反应器中重金属的含量，所有测试均使用超纯水作为进水合成废水，且所有反应器均安装在洁净棚内。通过凝胶包埋技术维持厌氧氨氧化生物质。在不存在硒（VI）和镍（II）的情况下，厌氧氨氧化反应器的启动量为18.9千克氮/（立方米载体·天）（每凝胶载体的氮转化率（NCR）），这表明维持厌氧氨氧化过程不需要或无需持续添加硒（VI）和镍（II）。在锌（II）受限的情况下，厌氧氨氧化过程无法启动，且氮转化率趋于迅速下降。重新添加0.005毫克/升的锌（II）后，氮转化率没有进一步下降，而是以非常缓慢的速度部分恢复。添加0.020毫克/升的锌（II）后，氮转化率完全恢复。这些结果表明，锌（II）受限会严重影响厌氧氨氧化过程的启动，而厌氧氨氧化过程不需要或无需持续添加硒（VI）和镍（II）。
