Wang Kun, Cheng Liang, He Benxiang
Key Laboratory of Sports Medicine, General Administration of Sport of China, Institute of Sports Medicine and Health, Chengdu Sport University, Chengdu, China.
Regen Ther. 2022 Mar 3;20:1-8. doi: 10.1016/j.reth.2022.02.001. eCollection 2022 Jun.
This study explored the effects and molecular mechanisms of asperosaponin VI in tendon disease.
Forty-eight purebred adult male New Zealand white rabbits were randomly divided into the normal group (normal, n = 8); saline group (saline, n = 8) and prostaglandin E2 group (n = 32), which was further divided into four subgroups that were treated with asperosaponin VI doses of 0 mg/kg (model, n = 8), 10 mg/kg (10, n = 8), 20 mg/kg (20, n = 8) and 40 mg/kg (40, n = 8). The expression levels of matrix metallopeptidase 1 (MMP1), metallopeptidase inhibitor 1 (TIMP1), transforming growth factor beta 1 (TGFB1), serpin family E member 1 (SERPINE1), collagen Ⅰ (COL1), collagen Ⅲ (COL3) and tenomodulin (TNMD) in Achilles tendon tissue were determined through Western blot analysis. The histopathological changes in tendon tissue were observed by using Masson staining and haematoxylin-eosin staining.
The expression levels of MMP1, TIMP1 and COL3 were higher and those of TGFB1, SERPINE1, COL1 and TNMD were lower in the 0 mg/kg group than in the normal group (P < 0.05). Compared with those in the 0 mg/kg group, the levels of MMP1 were lower in the 20 and 40 mg/kg groups. Compared with those in the 0 mg/kg group, the levels of TIMP1 were lower and the levels of TGFB1, COL1 and TNMD were higher in the 10, 20 and 40 mg/kg groups. In addition, compared with those in other groups, the levels of SERPINE1 in the 40 mg/kg group were significantly higher and the levels of COL3 in the 10 and 20 mg/kg groups were significantly lower (P < 0.05). Fibrous tissue arrangements and structures in the 40 mg/kg group were similar to those in the control group.
The effects of asperosaponin VI on injured tendons mainly involve eliminating inflammation, restoring balance to extracellular matrix collagen metabolism and inducing tendon cell proliferation. Asperosaponin VI is likely to be an ideal drug for the prevention and treatment of tendon disease.
本研究探讨了地锦草皂苷VI对肌腱疾病的影响及其分子机制。
将48只纯种成年雄性新西兰白兔随机分为正常组（正常，n = 8）；生理盐水组（生理盐水，n = 8）和前列腺素E2组（n = 32），后者进一步分为四个亚组，分别给予地锦草皂苷VI剂量为0 mg/kg（模型，n = 8）、10 mg/kg（10，n = 8）、20 mg/kg（20，n = 8）和40 mg/kg（40，n = 8）。通过蛋白质免疫印迹分析测定跟腱组织中基质金属蛋白酶1（MMP1）、金属蛋白酶抑制剂1（TIMP1）、转化生长因子β1（TGFB1）、丝氨酸蛋白酶抑制剂家族E成员1（SERPINE1）、胶原蛋白Ⅰ（COL1）、胶原蛋白Ⅲ（COL3）和腱调蛋白（TNMD）的表达水平。采用Masson染色和苏木精-伊红染色观察肌腱组织的组织病理学变化。
0 mg/kg组MMP1、TIMP1和COL3的表达水平高于正常组，TGFB1、SERPINE1、COL1和TNMD的表达水平低于正常组（P < 0.05）。与0 mg/kg组相比，20和40 mg/kg组MMP1水平较低。与0 mg/kg组相比，10、20和40 mg/kg组TIMP1水平较低，TGFB1、COL1和TNMD水平较高。此外，与其他组相比，40 mg/kg组SERPINE1水平显著较高，10和20 mg/kg组COL3水平显著较低（P < 0.05）。40 mg/kg组的纤维组织排列和结构与对照组相似。
地锦草皂苷VI对损伤肌腱的作用主要包括消除炎症、恢复细胞外基质胶原蛋白代谢平衡和诱导肌腱细胞增殖。地锦草皂苷VI可能是预防及治疗肌腱疾病的理想药物。
