• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Cloning and characterization of the gene encoding 3-deoxy-D-manno-octulosonate 8-phosphate synthetase from Escherichia coli.

作者信息

Woisetschläger M, Högenauer G

出版信息

J Bacteriol. 1986 Oct;168(1):437-9. doi: 10.1128/jb.168.1.437-439.1986.

DOI:10.1128/jb.168.1.437-439.1986
PMID:3531183
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC213473/
Abstract

The cloning of the gene for Escherichia coli PL-2 2-keto-3-deoxy-D-manno-octonate 8-phosphate synthetase is reported. Positive transformants showed an increase of approximately three-fold in specific activity of the enzyme both in E. coli and in Salmonella typhimurium as host cells. A subclone containing a 1.5-kilobase PvuII fragment overproduced active enzyme. Minicell experiments that allow the detection of plasmid encoded proteins revealed an insert-coded single protein band of 34 kilodaltons.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9161/213473/d32bc4d0d43c/jbacter00203-0448-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9161/213473/d32bc4d0d43c/jbacter00203-0448-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9161/213473/d32bc4d0d43c/jbacter00203-0448-a.jpg

相似文献

1
Cloning and characterization of the gene encoding 3-deoxy-D-manno-octulosonate 8-phosphate synthetase from Escherichia coli.
J Bacteriol. 1986 Oct;168(1):437-9. doi: 10.1128/jb.168.1.437-439.1986.
2
A single point mutation in 3-deoxy-D-manno-octulosonate-8-phosphate synthase is responsible for temperature sensitivity in a mutant strain of Salmonella typhimurium.
J Biol Chem. 2000 Oct 13;275(41):32141-6. doi: 10.1074/jbc.M001900200.
3
Molecular cloning, sequence analysis and functional characterization of the gene kdsA, encoding 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase of Chlamydia psittaci 6BC.鹦鹉热衣原体6BC株编码3-脱氧-D-甘露-2-辛酮糖酸-8-磷酸合酶的基因kdsA的分子克隆、序列分析及功能鉴定
Eur J Biochem. 1997 Feb 15;244(1):66-73. doi: 10.1111/j.1432-1033.1997.00066.x.
4
Regulation of the Salmonella typhimurium aroF gene in Escherichia coli.鼠伤寒沙门氏菌aroF基因在大肠杆菌中的调控
J Bacteriol. 1990 May;172(5):2259-66. doi: 10.1128/jb.172.5.2259-2266.1990.
5
Molecular cloning of the structural gene coding for CTP:CMP-3-deoxy-manno-octulosonate cytidylyltransferase from Escherichia coli K-12.来自大肠杆菌K-12的CTP：CMP-3-脱氧甘露糖辛酮酸胞苷酰转移酶编码结构基因的分子克隆。
J Bacteriol. 1985 Jul;163(1):256-61. doi: 10.1128/jb.163.1.256-261.1985.
6
Expression of genes kdsA and kdsB involved in 3-deoxy-D-manno-octulosonic acid metabolism and biosynthesis of enterobacterial lipopolysaccharide is growth phase regulated primarily at the transcriptional level in Escherichia coli K-12.参与3-脱氧-D-甘露糖辛酸代谢及肠杆菌脂多糖生物合成的基因kdsA和kdsB的表达在大肠杆菌K-12中主要在转录水平受到生长阶段的调控。
J Bacteriol. 1995 Aug;177(15):4488-500. doi: 10.1128/jb.177.15.4488-4500.1995.
7
The kdsA gene coding for 3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase is part of an operon in Escherichia coli.编码3-脱氧-D-甘露糖辛酸8-磷酸合成酶的kdsA基因是大肠杆菌中一个操纵子的一部分。
Mol Gen Genet. 1987 May;207(2-3):369-73. doi: 10.1007/BF00331603.
8
Relationship between cell death and altered lipid A synthesis in a temperature-sensitive lethal mutant of Salmonella typhimurium that is conditionally defective in 3-deoxy-D-manno-octulosonate-8-phosphate synthesis.鼠伤寒沙门氏菌温度敏感致死突变体中细胞死亡与脂质A合成改变之间的关系，该突变体在3-脱氧-D-甘露糖辛酮酸-8-磷酸合成方面存在条件性缺陷。
J Bacteriol. 1982 May;150(2):456-64. doi: 10.1128/jb.150.2.456-464.1982.
9
[Cloning of genes for 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase from methylotrophic Pseudomonas in Escherichia coli].[从甲基营养型假单胞菌中克隆3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶基因并在大肠杆菌中表达]
Genetika. 1990 Mar;26(3):418-23.
10
Cloning, expression, and biochemical characterization of 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P) synthase from the hyperthermophilic bacterium Aquifex pyrophilus.嗜热栖热菌3-脱氧-D-甘露-2-辛酮糖酸-8-磷酸（KDO8P）合酶的克隆、表达及生化特性分析
Extremophiles. 2003 Dec;7(6):471-81. doi: 10.1007/s00792-003-0346-3. Epub 2003 Aug 29.

引用本文的文献

1
Expression of genes kdsA and kdsB involved in 3-deoxy-D-manno-octulosonic acid metabolism and biosynthesis of enterobacterial lipopolysaccharide is growth phase regulated primarily at the transcriptional level in Escherichia coli K-12.参与3-脱氧-D-甘露糖辛酸代谢及肠杆菌脂多糖生物合成的基因kdsA和kdsB的表达在大肠杆菌K-12中主要在转录水平受到生长阶段的调控。
J Bacteriol. 1995 Aug;177(15):4488-500. doi: 10.1128/jb.177.15.4488-4500.1995.
2
Localization of the kdsA gene with the aid of the physical map of the Escherichia coli chromosome.借助大肠杆菌染色体物理图谱对kdsA基因进行定位。
J Bacteriol. 1988 Nov;170(11):5382-4. doi: 10.1128/jb.170.11.5382-5384.1988.
3

本文引用的文献

1
Purification and characterization of 3-deoxy-D-manno-octulosonate 8-phosphate synthetase from Escherichia coli.大肠杆菌3-脱氧-D-甘露糖辛酮酸8-磷酸合成酶的纯化与特性分析
J Bacteriol. 1980 Feb;141(2):635-44. doi: 10.1128/jb.141.2.635-644.1980.
2
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.从含有噬菌体SP6启动子的质粒中高效体外合成生物活性RNA和RNA杂交探针。
Nucleic Acids Res. 1984 Sep 25;12(18):7035-56. doi: 10.1093/nar/12.18.7035.
3
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
The kdsA gene coding for 3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase is part of an operon in Escherichia coli.
编码3-脱氧-D-甘露糖辛酸8-磷酸合成酶的kdsA基因是大肠杆菌中一个操纵子的一部分。
Mol Gen Genet. 1987 May;207(2-3):369-73. doi: 10.1007/BF00331603.
4
Isolation of Salmonella typhimurium strains that utilize exogenous 3-deoxy-D-manno-octulosonate for synthesis of lipopolysaccharide.利用外源性3-脱氧-D-甘露糖辛酮酸酯合成脂多糖的鼠伤寒沙门氏菌菌株的分离
J Bacteriol. 1987 Nov;169(11):5060-5. doi: 10.1128/jb.169.11.5060-5065.1987.
在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
4
Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.大肠杆菌中的超螺旋环状DNA-蛋白质复合物：纯化及诱导转化为开放环状DNA形式
Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66. doi: 10.1073/pnas.62.4.1159.
5
R factor proteins synthesized in Escherichia coli minicells: incorporation studies with different R factors and detection of deoxyribonucleic acid-binding proteins.在大肠杆菌微细胞中合成的R因子蛋白：不同R因子的掺入研究及脱氧核糖核酸结合蛋白的检测
J Bacteriol. 1974 Dec;120(3):1451-63. doi: 10.1128/jb.120.3.1451-1463.1974.
6
Isolation of a mutant of Salmonella typhimurium dependent on D-arabinose-5-phosphate for growth and synthesis of 3-deoxy-D-mannoctulosonate (ketodeoxyoctonate).鼠伤寒沙门氏菌突变体的分离，该突变体生长及合成3-脱氧-D-甘露辛酮糖酸（酮脱氧辛酮酸）依赖于D-阿拉伯糖-5-磷酸。
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3756-60. doi: 10.1073/pnas.69.12.3756.
7
Free flow electrophoresis as a tool for enrichment of mutants with temperature-dependent lethal mutations in lipid A synthesis.
Eur J Biochem. 1985 Apr 1;148(1):21-7. doi: 10.1111/j.1432-1033.1985.tb08801.x.
8
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.一种利用蛋白质 - 染料结合原理对微克级蛋白质进行定量的快速灵敏方法。
Anal Biochem. 1976 May 7;72:248-54. doi: 10.1016/0003-2697(76)90527-3.
9
Biosynthesis of lipid A. Enzymatic incorporation of 3-deoxy-D-mannooctulosonate into a precursor of lipid A in Salmonella typhimurium.
J Biol Chem. 1978 Mar 10;253(5):1503-11.
10
Isolation of mutants conditionally blocked in the biosynthesis of the 3-deoxy-D-manno-octulosonic-acid--lipid-A part of lipopolysaccharides derived from Salmonella typhimurium.
Eur J Biochem. 1977 Jun 1;76(1):41-9. doi: 10.1111/j.1432-1033.1977.tb11568.x.