Woisetschläger M, Högenauer G
Mol Gen Genet. 1987 May;207(2-3):369-73. doi: 10.1007/BF00331603.
The kdsA gene of Escherichia coli was sequenced. It consists of 284 codons and is the last gene of a larger transcription unit. The mRNA is terminated by a rho-independent termination signal with the potential to be active in both orientations. This region is followed by another termination signal which seems to be active in the opposite orientation. Upstream of the kdsA gene a second open reading frame (ORF) was found; both this and the kdsA gene are transcribed in the same mRNA molecule when coded from the chromosome. In a plasmid-carried insert transcription starts from a cryptic promoter within the ORF preceding the kdsA gene. This promoter is not active in the intact chromosome.
对大肠杆菌的kdsA基因进行了测序。它由284个密码子组成，是一个更大转录单元的最后一个基因。mRNA由一个不依赖于ρ因子的终止信号终止，该信号在两个方向上都可能是活跃的。该区域之后是另一个终止信号，它似乎在相反方向上是活跃的。在kdsA基因的上游发现了第二个开放阅读框（ORF）；当从染色体编码时，这个ORF和kdsA基因都在同一个mRNA分子中被转录。在质粒携带的插入片段中，转录从kdsA基因之前的ORF内的一个隐蔽启动子开始。这个启动子在完整的染色体中不活跃。
