鼠伤寒沙门氏菌突变体的分离，该突变体生长及合成3-脱氧-D-甘露辛酮糖酸（酮脱氧辛酮酸）依赖于D-阿拉伯糖-5-磷酸。
Isolation of a mutant of Salmonella typhimurium dependent on D-arabinose-5-phosphate for growth and synthesis of 3-deoxy-D-mannoctulosonate (ketodeoxyoctonate).
作者信息
Rick P D, Osborn M J
出版信息
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3756-60. doi: 10.1073/pnas.69.12.3756.
Abstract
A new type of auxotrophic mutant of Salmonella typhimurium has been isolated that is defective in the synthesis of the 3-deoxy-D-mannooctulosonate (ketodeoxyoctonate) region of the lipopolysaccharide and requires D-arabinose-5-phosphate for growth. Genetic and biochemical evidence indicated that the mutant defect was due to an altered ketodeoxyoctonate-8-phosphate synthetase with an apparent K(m) for D-arabinose-5-phosphate 35-fold higher than that of the parental enzyme. As a result of this enzymatic lesion, the mutant strain was dependent on exogenous D-arabinose-5-phosphate both for growth and for synthesis of a complete lipopolysaccharide.
摘要
已分离出一种新型的鼠伤寒沙门氏菌营养缺陷型突变体，该突变体在脂多糖的3-脱氧-D-甘露辛酮酸（酮脱氧辛酮酸）区域合成方面存在缺陷，并且生长需要D-阿拉伯糖-5-磷酸。遗传和生化证据表明，该突变体的缺陷是由于酮脱氧辛酮酸-8-磷酸合成酶发生改变，其对D-阿拉伯糖-5-磷酸的表观K(m)值比亲本酶高35倍。由于这种酶损伤，突变菌株在生长和合成完整脂多糖方面都依赖于外源性D-阿拉伯糖-5-磷酸。
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