Wildeman A G, Rasquinha I, Nazar R N
J Biol Chem. 1986 Oct 15;261(29):13401-3.
An oligonucleotide probe was used to isolate yeast genomic clones containing DNA sequences with repetitive elements consisting primarily of a tandemly arranged trinucleotide, CAT. Hybridization analyses estimate that the yeast genome contains 40-50 CAT clusters, representing the first repetitive DNA sequence family found in yeast. Sequence analyses show short spacers between the CAT repeats consisting of closely related trinucleotides, primarily CGT. Some of the CAT clusters are located in longer repeating elements with lengths of 7 nucleotides or more. In one case a three-times-repeated 27-nucleotide sequence bears striking homology to the 21-base pair repeat region of the mammalian simian virus 40 promoter element. Hybridization studies further suggest that the "CAT" sequences may be widely dispersed in many diverse organisms including Escherichia coli, Drosophila, and man.
使用寡核苷酸探针分离酵母基因组克隆，这些克隆包含主要由串联排列的三核苷酸CAT组成的具有重复元件的DNA序列。杂交分析估计酵母基因组包含40 - 50个CAT簇，这代表了在酵母中发现的第一个重复DNA序列家族。序列分析表明，CAT重复序列之间存在由密切相关的三核苷酸（主要是CGT）组成的短间隔序列。一些CAT簇位于长度为7个核苷酸或更长的较长重复元件中。在一个案例中，一个重复三次的27个核苷酸的序列与哺乳动物猿猴病毒40启动子元件的21个碱基对重复区域具有显著的同源性。杂交研究进一步表明，“CAT”序列可能广泛分布于包括大肠杆菌、果蝇和人类在内的许多不同生物中。
