Horowitz H, Haber J E
Nucleic Acids Res. 1984 Sep 25;12(18):7105-21. doi: 10.1093/nar/12.18.7105.
We have determined the nucleotide sequence of a region of DNA derived from the end of one chromosome of the yeast, Saccharomyces cerevisiae. Inspection of the sequence reveals the presence of 12 tandem direct repeats, each 36 nucleotides long and having nearly identical sequence. Each 36 base-pair repeat can be further subdivided into three tandem sub-repeats of a similar 12 base-pair sequence. Analysis of total genomic yeast DNA from several strains by Southern hybridization suggests that the number of tandem 36 base-pair repeat units may vary from approximately 8 to 25 among different telomeric regions. Differences in the number of repeats may have arisen by unequal crossing over between them. Furthermore, the finding that the pattern of bases at multiple variable positions within the repeat unit is not random suggests that these regions may undergo gene conversion events that render them homogeneous.
我们已经确定了来自酿酒酵母（Saccharomyces cerevisiae）一条染色体末端的一段DNA区域的核苷酸序列。对该序列的检查发现存在12个串联直接重复序列，每个重复序列长36个核苷酸，且具有几乎相同的序列。每个36个碱基对的重复序列可进一步细分为三个类似的12个碱基对序列的串联亚重复序列。通过Southern杂交对几个菌株的总基因组酵母DNA进行分析表明，在不同的端粒区域中，串联36个碱基对重复单元的数量可能在大约8到25之间变化。重复序列数量的差异可能是由于它们之间的不等交换产生的。此外，重复单元内多个可变位置的碱基模式并非随机这一发现表明，这些区域可能经历基因转换事件，从而使其变得均匀一致。
