Saxon A, Mitsuyasu R, Stevens R, Champlin R E, Kimata H, Gale R P
J Clin Invest. 1986 Oct;78(4):959-67. doi: 10.1172/JCI112686.
Bone marrow transplant donors were immunized with tetanus/diphtheria toxoids 6-7 d before bone marrow donation to investigate the role of B cell subpopulations in reconstitution of humoral immunity. Lymphoblastoid B cells spontaneously producing IgG antitetanus and/or antidiphtheria toxoid were detected in the donor marrows at the time of transplantation. Recipients rapidly demonstrated 3-90-fold increases in serum IgG antitetanus and antidiphtheria toxoid levels. Antidiphtheria fragment A antibody in three donor/recipient pairs demonstrated spectrotypic identity indicating transfer of the donors' response. Reimmunization of three recipients 64-154 d after transplant revealed an IgG antibody response associated with reappearance of spontaneous antibody-producing B cells and an antidiphtheria fragment A response characteristics of the donor's immune response. These observations extend the understanding of the role of B cell subpopulations and provide a basis for specific modulation of immunity in the setting of bone marrow transplantation.
在骨髓捐献前6 - 7天，对骨髓移植供体进行破伤风/白喉类毒素免疫，以研究B细胞亚群在体液免疫重建中的作用。在移植时，在供体骨髓中检测到自发产生IgG抗破伤风和/或抗白喉类毒素的淋巴母细胞样B细胞。受体血清中抗破伤风和抗白喉类毒素的IgG水平迅速升高了3 - 90倍。三对供体/受体中的抗白喉A片段抗体表现出光谱型一致性，表明供体的反应发生了转移。对三名受体在移植后64 - 154天进行再次免疫，结果显示IgG抗体反应与自发产生抗体的B细胞再次出现以及供体免疫反应特征的抗白喉A片段反应相关。这些观察结果扩展了对B细胞亚群作用的理解，并为在骨髓移植情况下特异性调节免疫提供了基础。
