Marshall N A, Chapman M D, Saxon A
J Allergy Clin Immunol. 1986 Sep;78(3 Pt 1):430-5. doi: 10.1016/0091-6749(86)90029-1.
We investigated allergenic cross-reactivity among species of the blood-feeding insects of the subfamily Triatominae. By skin testing, patients allergic to either Triatoma protracta or T. rubida gave positive responses only to the respective salivary antigen. RAST-inhibition experiments demonstrated that binding of IgE antibodies to T. protracta antigen was not inhibited by salivary extracts from T. rubida, T. cavernicola, T. rubrofasciata, or Rhodnius prolixus. The same level of species specificity was found for IgE antibodies to T. rubida. By direct RAST, no T. rubida positive serum bound T. protracta antigen, and 29 of 30 T. protracta positive sera failed to bind T. rubida. One serum from a T. protracta-allergic patient contained IgE antibodies to both T. protracta and T. rubida. RAST-inhibition experiments demonstrated that these antibodies did not cross-react and that this person had separate species-specific antibodies to T. protracta and T. rubida antigens. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of salivary extracts demonstrated that the lower molecular weight bands that contain the antigens responsible for human allergic reactions differed in number and size in all species tested. These studies demonstrate species specificity for the allergic response to Triatoma and stress the importance of accurate insect identification and the need for species-specific antigens for diagnosis and immunotherapy.
我们研究了锥蝽亚科吸血昆虫物种间的变应原交叉反应性。通过皮肤试验，对长红锥蝽或红带锥蝽过敏的患者仅对相应的唾液抗原产生阳性反应。放射变应原吸附试验（RAST）抑制实验表明，红带锥蝽、洞穴锥蝽、红边锥蝽或南美锥蝽的唾液提取物均未抑制IgE抗体与长红锥蝽抗原的结合。对于红带锥蝽的IgE抗体也发现了相同程度的物种特异性。通过直接RAST检测，没有红带锥蝽阳性血清能结合长红锥蝽抗原，30份长红锥蝽阳性血清中有29份不能结合红带锥蝽抗原。一份来自长红锥蝽过敏患者的血清含有针对长红锥蝽和红带锥蝽的IgE抗体。RAST抑制实验表明，这些抗体不会发生交叉反应，且该患者针对长红锥蝽和红带锥蝽抗原具有单独的物种特异性抗体。唾液提取物的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析表明，在所有测试物种中，含有引发人类过敏反应抗原的较低分子量条带在数量和大小上存在差异。这些研究证明了对锥蝽过敏反应的物种特异性，并强调了准确鉴定昆虫的重要性以及诊断和免疫治疗中使用物种特异性抗原的必要性。
