Section of Vector Biology, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, 12735 Twinbrook Parkway room 2E32D, Rockville, MD 20852, USA.
J Med Entomol. 2012 May;49(3):563-72. doi: 10.1603/me11243.
The kissing bug Triatoma rubida (Uhler, 1894) is found in southwestern United States and parts of Mexico where it is found infected with Trypanosoma cruzi, invades human dwellings and causes allergies from their bites. Although the protein salivary composition of several triatomine species is known, not a single salivary protein sequence is known from T. rubida. Furthermore, the salivary diversity of related hematophagous arthropods is very large probably because of the immune pressure from their hosts. Here we report the sialotranscriptome analysis of T. rubida based on the assembly of 1,820 high-quality expressed sequence tags, 51% of which code for putative secreted peptides, including lipocalins, members of the antigen five family, apyrase, hemolysin, and trialysin families. Interestingly, T. rubida lipocalins are at best 40% identical in primary sequence to those of T. protracta, a kissing bug that overlaps its range with T. rubida, indicating the diversity of the salivary lipocalins among species of the same hematophagous genus. We additionally found several expressed sequence tags coding for proteins of clear Trypanosoma spp. origin. This work contributes to the future development of markers of human and pet exposure to T. rubida and to the possible development of desensitization therapies. Supp. Data 1 and 2 (online only) of the transcriptome and deducted protein sequences can be obtained from http://exon.niaid.nih.gov/transcriptome/Trubida/Triru-S1-web.xlsx and http://exon.niaid.nih.gov/transcriptome/Trubida/Triru-S2-web.xlsx.
吻虫 Triatoma rubida（Uhler，1894）分布于美国西南部和墨西哥部分地区，感染克氏锥虫，入侵人类住所并因其叮咬引起过敏。尽管已经了解几种三锥虫物种的唾液蛋白组成，但尚未从 T. rubida 中鉴定出单一的唾液蛋白序列。此外，相关吸血节肢动物的唾液多样性非常大，可能是由于来自宿主的免疫压力。在这里，我们根据 1820 个高质量表达序列标签的组装报告了 T. rubida 的唾液转录组分析，其中 51%的序列编码假定的分泌肽，包括脂联素、抗原五家族成员、apyrase、hemolysin 和 trialysin 家族。有趣的是，T. rubida 的脂联素在一级序列上与 T. protracta 的脂联素最多只有 40%的同源性，T. protracta 与 T. rubida 的分布重叠，表明同一吸血属的物种之间唾液脂联素的多样性。我们还发现了几个表达序列标签，编码明显的 Trypanosoma spp. 起源的蛋白质。这项工作有助于未来开发人类和宠物接触 T. rubida 的标志物，并可能开发脱敏疗法。转录组和推导蛋白序列的补充数据 1 和 2（仅在线）可从 http://exon.niaid.nih.gov/transcriptome/Trubida/Triru-S1-web.xlsx 和 http://exon.niaid.nih.gov/transcriptome/Trubida/Triru-S2-web.xlsx 获得。
