Li Lin, Uppal Timsy, Hartley Paul D, Gorzalski Andrew, Pandori Mark, Picker Michael A, Verma Subhash C, Pagilla Krishna
University of Nevada.
University of Nevada, Reno School of Medicine.
Res Sq. 2022 Mar 17:rs.3.rs-1435729. doi: 10.21203/rs.3.rs-1435729/v1.
Detection of SARS-CoV-2 viral load in wastewater has been highly informative in estimating the approximate number of infected individuals in the surrounding communities. Recent developments in wastewater monitoring to determine community prevalence of COVID-19 further extends into identifying SARS-CoV-2 variants, including those being monitored for having enhanced transmissibility. We sequenced genomic RNA derived from wastewater to determine the variants of coronaviruses circulating in the communities. Wastewater samples were collected from Truckee Meadows Water Reclamation Facility (TMWRF) from November 2021 to June 2021 were analyzed for SARS-CoV-2 variants and were compared with the variants detected in the clinical specimens (nasal/nasopharyngeal swabs) of infected individuals during the same period. The comparison was found to be conclusively in agreement. Therefore, wastewater monitoring for SARS-CoV-2 variants in the community is a feasible strategy both as a complementary tool to clinical specimen testing and in the latter's absence.
检测废水中的新冠病毒载量对于估算周边社区受感染个体的大致数量具有重要意义。废水监测领域的最新进展旨在确定新冠病毒在社区中的流行情况，这进一步扩展到识别新冠病毒变异株，包括那些因传播性增强而受到监测的变异株。我们对源自废水的基因组RNA进行测序，以确定社区中传播的冠状病毒变异株。收集了特拉基草甸水回收设施（TMWRF）在2021年11月至2021年6月期间的废水样本，分析其中的新冠病毒变异株，并与同期在感染个体的临床标本（鼻拭子/鼻咽拭子）中检测到的变异株进行比较。结果发现两者完全一致。因此，对社区中的新冠病毒变异株进行废水监测，无论是作为临床标本检测的补充工具，还是在缺乏临床标本检测的情况下，都是一种可行的策略。
