Naninck Thibaut, Kahlaoui Nidhal, Lemaitre Julien, Maisonnasse Pauline, De Mori Antoine, Pascal Quentin, Contreras Vanessa, Marlin Romain, Relouzat Francis, Delache Benoît, Hérate Cécile, Aldon Yoann, van Gils Marit, Zabaleta Nerea, Ho Tsong Fang Raphaël, Bosquet Nathalie, Sanders Rogier W, Vandenberghe Luk H, Chapon Catherine, Le Grand Roger
Université Paris-Saclay, Inserm, CEA, Center for Immunology of Viral, Auto-immune, Hematological and Bacterial Diseases (IMVA-HB/IDMIT), Fontenay-aux-Roses & Le Kremlin-Bicêtre, Paris, France.
Departments of Medical Microbiology of the Amsterdam UMC, Amsterdam Institute for Infection and Immunity, University of Amsterdam, 1105 Amsterdam, the Netherlands.
iScience. 2022 Apr 15;25(4):104101. doi: 10.1016/j.isci.2022.104101. Epub 2022 Mar 17.
Non-human primates (NHPs) are particularly relevant as preclinical models for SARS-CoV-2 infection and nuclear imaging may represent a valuable tool for monitoring infection in this species. We investigated the benefit of computed X-ray tomography (CT) and [F]-FDG positron emission tomography (PET) to monitor the early phase of the disease in a large cohort (n = 76) of SARS-CoV-2 infected macaques. Following infection, animals showed mild COVID-19 symptoms including typical lung lesions. CT scores at the acute phase reflect the heterogeneity of lung burden following infection. Moreover, [F]-FDG PET revealed that FDG uptake was significantly higher in the lungs, nasal cavities, lung-draining lymph nodes, and spleen of NHPs by 5 days postinfection compared to pre-infection levels, indicating early local inflammation. The comparison of CT and PET data from previous COVID-19 treatments or vaccines we tested in NHP, to this large cohort of untreated animals demonstrated the value of imaging in preclinical trials.
非人灵长类动物（NHPs）作为严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染的临床前模型具有特殊意义，而核成像可能是监测该物种感染情况的一种有价值的工具。我们研究了计算机X线断层扫描（CT）和[F] -氟代脱氧葡萄糖正电子发射断层扫描（PET）在一大群（n = 76）感染SARS-CoV-2的猕猴中监测疾病早期阶段的益处。感染后，动物出现了包括典型肺部病变在内的轻度新型冠状病毒肺炎（COVID-19）症状。急性期的CT评分反映了感染后肺部负担的异质性。此外，[F] -FDG PET显示，与感染前水平相比，感染后5天NHPs的肺部、鼻腔、引流肺的淋巴结和脾脏中的FDG摄取显著更高，表明早期局部炎症。将我们在NHPs中测试的先前COVID-19治疗方法或疫苗的CT和PET数据与这一大群未治疗动物的数据进行比较，证明了成像在临床前试验中的价值。
