Department of Population Sciences, Beckman Research Institute, City of Hope, 1500 E. Duarte Road, Duarte, CA 91010, USA.
Center for Cancer and Aging Research, City of Hope, 1500 E. Duarte Road, Duarte, CA 91010, USA.
STAR Protoc. 2022 Mar 16;3(2):101182. doi: 10.1016/j.xpro.2022.101182. eCollection 2022 Jun 17.
Dysregulation of the transcriptional or translational machinery can alter the stoichiometry of multiprotein complexes and occurs in natural processes such as aging. Loss of stoichiometry has been shown to alter protein complex functions. We provide a protocol and associated code that use omics data to quantify these stoichiometric changes via statistical dispersion utilizing the interquartile range of expression values per grouping variable. This descriptive statistical approach enables the quantification of stoichiometry changes without additional data acquisition. For complete details on the use and execution of this protocol, please refer to Hinz et al. (2021).
转录或翻译机制的失调会改变多蛋白复合物的化学计量，并发生在衰老等自然过程中。已经表明，化学计量的损失会改变蛋白质复合物的功能。我们提供了一个协议和相关的代码，利用组学数据通过利用每个分组变量的表达值的四分位距来通过统计离散度来量化这些化学计量变化。这种描述性统计方法能够在不进行额外数据采集的情况下定量化学计量变化。有关此协议的使用和执行的完整详细信息，请参阅 Hinz 等人。（2021 年）。
