使用四通道记录系统评估小鼠听觉脑干反应的方案。

Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.

Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China.

STAR Protoc. 2022 Mar 16;3(2):101251. doi: 10.1016/j.xpro.2022.101251. eCollection 2022 Jun 17.

Evaluating auditory sensitivity is critical to hearing research, particularly to that focusing on hearing impairment. Auditory brainstem response recording is frequently used in mice to assess auditory sensitivity and is an approach superior to the traditional techniques. Here, we describe a protocol for recording ABR in mice using four-channel equipment. We detail the procedures of animal preparation, the setup of the ABR recording system, the click- and tone burst-evoked ABR recordings, and data analysis.

评估听觉敏感性对于听力研究至关重要，特别是对于关注听力障碍的研究。听觉脑干反应记录在小鼠中常用于评估听觉敏感性，是一种优于传统技术的方法。在这里，我们描述了使用四通道设备记录小鼠 ABR 的方案。我们详细介绍了动物准备、ABR 记录系统设置、点击和啁啾诱发 ABR 记录以及数据分析的过程。