Department of Physics, Jadavpur University, Kolkata, India.
School of Pharmaceutical Sciences, Siksha 'O' Anusandhan (Deemed to be University), Bhubaneswar, India.
J Microencapsul. 2022 Mar;39(2):136-144. doi: 10.1080/02652048.2022.2053757. Epub 2022 Apr 6.
Docetaxel (DTX) loaded bio-compatible PLGA-PEG encapsulated zinc ferrite nanoparticles (ZFNP) formulation was developed and evaluated against C6 glioma cells.
The ZFNP were characterised using XRD, FE-SEM, TEM, etc. A series of drug formulations were fabricated by conjugating hydrothermally synthesised ZFNP with DTX in a PLGA-PEG matrix and optimised for drug loading. FTIR and DLS analysis of the formulation along with drug release, cytotoxicity, cellular uptake, and haemolytic effect were evaluated.
Spherical, monodisperse, crystalline ZFNP with an average size of ∼28 nm were formed. The optimised formulation showed a hydrodynamic diameter of ∼147 nm, a surface charge of -34.8 mV, a drug loading of 6.9% (w/w) with prolonged drug release properties, and higher toxicity in C6 glioma cells compared to free DTX along with good internalisation and negligible haemolysis.
The results indicate ZFNP could be effectively used as nanodrug carrier for delivery of docetaxel to glioma cells.
制备并评价载多西紫杉醇（DTX）的生物相容性 PLGA-PEG 包裹的锌铁氧体纳米粒子（ZFNP）制剂对 C6 神经胶质瘤细胞的作用。
采用 XRD、FE-SEM、TEM 等方法对 ZFNP 进行了表征。通过将水热法合成的 ZFNP 与 DTX 偶联到 PLGA-PEG 基质中，制备了一系列药物制剂，并对其进行了药物载药量的优化。对制剂进行了傅里叶变换红外光谱（FTIR）和动态光散射（DLS）分析以及药物释放、细胞毒性、细胞摄取和溶血效应的评估。
形成了具有约 28nm 平均粒径的球形、单分散、结晶 ZFNP。优化后的制剂表现出约 147nm 的水动力直径、-34.8mV 的表面电荷、6.9%（w/w）的药物载药量，具有延长的药物释放特性，与游离 DTX 相比，对 C6 神经胶质瘤细胞的毒性更高，同时具有良好的内化作用和可忽略的溶血作用。
结果表明，ZFNP 可有效用作递送至神经胶质瘤细胞的多西紫杉醇的纳米药物载体。
