鉴定感染口蹄疫病毒的 PK-15 细胞中差异表达的环状 RNA 并进行功能分析。

Profiling and functional analysis of differentially expressed circular RNAs identified in foot-and-mouth disease virus infected PK-15 cells.

机构信息

State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.

出版信息

Vet Res. 2022 Mar 21;53(1):24. doi: 10.1186/s13567-022-01037-w.

DOI:10.1186/s13567-022-01037-w

PMID:35313983

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8935690/

Abstract

Circular RNAs (circRNAs) are a new type of endogenous noncoding RNA that exhibit a variety of biological functions. However, it is not clear whether they are involved in foot-and-mouth disease virus (FMDV) infection and host response. In this study, we established circRNA expression profiles in FMDV-infected PK-15 cells using RNA-seq (RNA-sequencing) technology analysis. The biological function of the differentially expressed circRNAs was determined by protein interaction network, Gene Ontology (GO), and Kyoto Encyclopedia of Gene and Genome (KEGG) pathway enrichment. We found 1100 differentially expressed circRNAs (675 downregulated and 425 upregulated) which were involved in various biological processes such as protein ubiquitination modification, cell cycle regulation, RNA transport, and autophagy. We also found that circRNAs identified after FMDV infection may be involved in the host cell immune response. RNA-Seq results were validated by circRNAs qRT-PCR. In this study, we analyzed for the first time circRNAs expression profile and the biological function of these genes after FMDV infection of host cells. The results provide new insights into the interactions between FMDV and host cells.

摘要

环状 RNA（circRNAs）是一种新型的内源性非编码 RNA，具有多种生物学功能。然而，目前尚不清楚它们是否参与了口蹄疫病毒（FMDV）感染和宿主反应。在本研究中，我们使用 RNA-seq（RNA 测序）技术分析建立了 FMDV 感染 PK-15 细胞中的 circRNA 表达谱。通过蛋白互作网络、基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路富集分析，确定了差异表达 circRNAs 的生物学功能。我们发现了 1100 个差异表达的 circRNAs（675 个下调和 425 个上调），它们参与了多种生物学过程，如蛋白泛素化修饰、细胞周期调控、RNA 转运和自噬。我们还发现，FMDV 感染后鉴定的 circRNAs 可能参与了宿主细胞免疫反应。circRNAs qRT-PCR 验证了 RNA-Seq 结果。在本研究中，我们首次分析了 FMDV 感染宿主细胞后 circRNAs 的表达谱和这些基因的生物学功能。研究结果为 FMDV 与宿主细胞的相互作用提供了新的见解。