College of Environmental Science and Engineering, Qilu University of Technology (Shandong Academy of Science), Jinan 250353, PR China.
College of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, PR China.
Bioresour Technol. 2022 May;351:127027. doi: 10.1016/j.biortech.2022.127027. Epub 2022 Mar 18.
Low hydrogen (H) yield via dark fermentation often occurs, being mainly due to H generation pathway shift. In this study, lanthanum-iron oxide nanoparticles (LaFeO NPs) were prepared to investigate their effects on bioH production. The highest H yield of 289.8 mL/g glucose was found at 100 mg/L of LaFeO, being 47.6% higher than that from the control (196.3 mL/g glucose). The relative abundance of Firmicutes increased from 54.2% to 67.5%. The large specific surface area of LaFeO provided sufficient sites for the colonization of Firmicutes and increased the bacterial access to nutrients. Additionally, the La gradually released from LaFeO NPs raised microbial transmembrane transport capacity, promoting glycolytic efficiency and Fe availability, thereby increasing hydrogenase content, and shifting the bioH evolution to butyrate pathway for more H. This provides the novelty for biochemical utilization of La and new insights into the improved H yield amended with LaFeO.
低氢（H）产率通过黑暗发酵经常发生，主要是由于 H 生成途径的转变。在这项研究中，制备了镧铁氧化物纳米粒子（LaFeO NPs），以研究它们对生物 H 生产的影响。在 100mg/L LaFeO 下，发现 H 的最高产率为 289.8mL/g 葡萄糖，比对照（196.3mL/g 葡萄糖）高 47.6%。厚壁菌门的相对丰度从 54.2%增加到 67.5%。LaFeO 的大比表面积为厚壁菌门的定殖提供了足够的位点，并增加了细菌对营养物质的获取。此外，LaFeO NPs 中逐渐释放的 La 提高了微生物跨膜运输能力，促进了糖酵解效率和 Fe 的可用性，从而增加了氢化酶的含量，并将生物 H 的演化转移到丁酸途径以产生更多的 H。这为 La 的生物化学利用提供了新颖性，并为用 LaFeO 提高 H 产量提供了新的见解。
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