College of Environmental Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.
Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China.
Bioresour Technol. 2022 Oct;361:127676. doi: 10.1016/j.biortech.2022.127676. Epub 2022 Jul 22.
Dark fermentation (DF) is a green hydrogen (H) production process, but it is far below the theoretical H yield. In this study, calcium ferrite nanoparticles (CaFeO NPs) were produced to augment H yield via DF. The highest H yield of 250.1 ± 6.5 mL/g glucose was achieved at 100 mg/L CaFeO NPs. Furtherincreasein CaFeO NPs above 100 mg/L, such as 600 mg/L, would slightly lower H yield to 208.6 ± 2.6 mL/g glucose. The CaFeO NPs in DF system released calcium and iron ions, promoting granular sludge formation andDF microbial activity. Soluble metabolites revealed that butyric acid was raised by CaFeO NPs, which indicated the improved metabolic pathway for more H. Microbial structure composition further illustrated that CaFeO NPs could increase the abundance of dominant microbial populations, with the supremacy of Firmicutes up to 71.22 % in the bioH evolution group augmented with 100 mg/L CaFeO NPs.
黑暗发酵（DF）是一种绿色制氢（H）的方法，但它的产氢率远低于理论值。在本研究中，通过 DF 利用铁酸钙纳米颗粒（CaFeO NPs）来提高 H 的产量。在 100mg/L CaFeO NPs 时，H 的最高产量达到 250.1±6.5mL/g 葡萄糖。进一步增加 CaFeO NPs 浓度（如 600mg/L），H 的产量会略有下降至 208.6±2.6mL/g 葡萄糖。DF 系统中的 CaFeO NPs 释放出钙和铁离子，促进颗粒污泥的形成和 DF 微生物的活性。可溶性代谢物表明，CaFeO NPs 提高了丁酸的含量，这表明改善了代谢途径，从而产生了更多的 H。微生物结构组成进一步表明，CaFeO NPs 可以增加优势微生物种群的丰度，在添加 100mg/L CaFeO NPs 的生物 H 演化组中，厚壁菌门的丰度高达 71.22%。
