College of Environmental Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.
College of Environmental Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.
Bioresour Technol. 2023 Oct;386:129506. doi: 10.1016/j.biortech.2023.129506. Epub 2023 Jul 17.
For low yield in dark fermentation (DF), in this study, the carbonized humic acid (CHA) was produced and added to DF for enhancing biohydrogen (bioH) yield at mesophilic condition. The highest bioH yield was 151.08 mL/g glucose with the addition of CHA at 80 mg/L, which was 35.27% and 16.53% higher than those of 0 mg/L CHA and 80 mg/L mineral humic acid (MHA) groups, respectively. Electrons preferentially conducted via the butyrate pathway due to CHA amendments, which corresponded to the prediction of relevant functional genes. Furthermore, CHA possessed distinctive advantages over MHA, which acted as an electron shuttle to facilitate electron transfer, released metal ions as an essential signal mediator and favored the reduction of ferredoxin, obtaining more H. The use of CHA in the field of H-DF depicted the high-value utilization and industrial chain extension of MHA.
针对黑暗发酵（DF）中产量低的问题，本研究制备了碳化腐殖酸（CHA）并将其添加到 DF 中，以在中温条件下提高生物氢（bioH）产量。添加 80mg/L CHA 时，bioH 的最高产量为 151.08mL/g 葡萄糖，分别比 0mg/L CHA 组和 80mg/L 矿物腐殖酸（MHA）组高 35.27%和 16.53%。由于 CHA 的添加，电子优先通过丁酸途径进行传导，这与相关功能基因的预测结果一致。此外，CHA 比 MHA 具有独特的优势，因为它可以作为电子穿梭体促进电子转移，释放金属离子作为必需的信号介质，并有利于铁氧还蛋白的还原，从而获得更多的 H。CHA 在 H-DF 领域的应用描绘了 MHA 的高附加值利用和产业链延伸。
