Li Zhenmin, Wang Jiangmei, Tian Kexin, Zhou Chen, Pei Yong, Zhang Jishi, Zang Lihua
College of Environmental Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan250353, China.
Shandong Weifang Ecological Environment Monitoring Center, Weifang261041, China.
ACS Omega. 2022 Nov 1;7(45):41594-41605. doi: 10.1021/acsomega.2c05580. eCollection 2022 Nov 15.
The positive effects of metal oxide nanoparticles (NPs) on dark fermentation (DF) for biohydrogen synthesis have been increased, and the mechanism still needs to be further revealed. In this study, nickel-cobalt oxide (NiCoO) NPs were prepared to increase H yield via DF. The highest (259.67 mL/g glucose) and the lowest (188.14 mL/g glucose) yields were achieved at 400 and 800 mg/L NiCoO NPs added, respectively, with their corresponding 33.97% increase and 2.93% decrease compared with the control yield (193.82 mL/g glucose). Meanwhile, the microbial community further confirmed that NiCoO NPs increased the abundance of the dominant H-producing by 23.05%. The gene prediction also showed that NiCoO NPs increased the abundance of genes encoding the rate-limiting enzyme pyruvate kinase in glycolysis, thus increasing the substrate conversion. Moreover, the gene abundance of key enzymes directly related to H evolution was also increased at different levels.
金属氧化物纳米颗粒（NPs）对生物制氢的暗发酵（DF）的积极作用有所增强，但其作用机制仍需进一步揭示。在本研究中，制备了镍钴氧化物（NiCoO）纳米颗粒以通过暗发酵提高氢气产量。分别在添加400和800 mg/L NiCoO纳米颗粒时获得了最高（259.67 mL/g葡萄糖）和最低（188.14 mL/g葡萄糖）产量，与对照产量（193.82 mL/g葡萄糖）相比，它们分别相应增加了33.97%和降低了2.93%。同时，微生物群落进一步证实，NiCoO纳米颗粒使主要产氢菌的丰度增加了23.05%。基因预测还表明，NiCoO纳米颗粒增加了糖酵解中限速酶丙酮酸激酶编码基因的丰度，从而提高了底物转化率。此外，与产氢直接相关的关键酶的基因丰度也在不同程度上有所增加。
