Mu Di, Jiang Yu, He Jing, Zhang Yuanxing, Yang Dahai, Liu Qin, Wang Zhuang
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, 519000, China; Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai, 200237, China.
Fish Shellfish Immunol. 2022 May;124:273-279. doi: 10.1016/j.fsi.2022.03.016. Epub 2022 Mar 18.
Propolis is non-hazardous resinous substance mixture containing bioactive ingredients such as polyphenols, flavonoids and organic acid. It has been widely used as food supplement and immune adjuvant due to its benefits in anti-microbial and immunomodulation. Edwardsiella piscicida is a kind of threatening pathogen which could cause high mortality in turbot. However, whether propolis could enhance the innate immune response against E. piscicida infection in turbot remains unknown. In this study, we found dietary propolis addition could improve the expression of anti-oxidative stress related enzymes, e.g., SOD, CAT and GPT, and relieved the histopathological changes of juvenile turbot after E. piscicida infection. Moreover, propolis addition increased the expression of cytokines such as il-1β, il-6 and tnf-α in different organs of juvenile turbot. Importantly, rescued survival and decreased bacterial loads were observed in propolis feeding group. Taken together, these findings suggest that the important roles of propolis in protecting juvenile turbot from E. piscicida infection, indicating propolis might be applied as a promising immunopotentiator candidate in aquaculture.
蜂胶是一种无毒的树脂类物质混合物，含有多酚、黄酮类化合物和有机酸等生物活性成分。由于其在抗菌和免疫调节方面的益处，它已被广泛用作食品补充剂和免疫佐剂。杀鱼爱德华氏菌是一种具有威胁性的病原体，可导致大菱鲆的高死亡率。然而，蜂胶是否能增强大菱鲆对杀鱼爱德华氏菌感染的先天免疫反应仍不清楚。在本研究中，我们发现添加膳食蜂胶可以提高抗氧化应激相关酶（如超氧化物歧化酶、过氧化氢酶和谷丙转氨酶）的表达，并减轻杀鱼爱德华氏菌感染后幼鱼的组织病理学变化。此外，添加蜂胶增加了幼鱼不同器官中白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α等细胞因子的表达。重要的是，在喂食蜂胶的组中观察到存活率提高和细菌载量降低。综上所述，这些发现表明蜂胶在保护幼鱼免受杀鱼爱德华氏菌感染方面具有重要作用，表明蜂胶可能作为水产养殖中有前景的免疫增强剂候选物应用。
