State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai 200237, China.
Microbiol Res. 2022 Oct;263:127043. doi: 10.1016/j.micres.2022.127043. Epub 2022 Apr 30.
The spread of multi-drug resistant (MDR) bacteria has posed a threat to the development of aquaculture. Due to its effective bactericidal ability, phage therapy has been considered as an alternative to antibiotics to reduce infection caused by MDR bacteria. In this study, two Edwardsiella piscicida phages were newly-isolated and characterized to prevent or treat infection in aquaculture. The phages were designated as vB_EpM_ZHS and vB_EpP_ZHX belonging to Myoviridae and Podoviridae families, respectively, in terms of genome sequence and morphology analyses. The combination of vB_EpM_ZHS and vB_EpP_ZHX improved the therapeutic efficacy both in vitro and in vivo. The phage cocktail significantly inhibited bacterial growth in vitro and decreased approximately 40% of mortality rate and an order of magnitude of bacterial burden in zebrafish and turbot infected by E. piscicida. Moreover, the phage cocktail increased transcription levels of tumor necrosis factor-alpha (TNF-α), interleukin-12 (IL-12), interleukin-6 (IL-6), and interleukin-1β (IL-1β) and alleviated inflammatory levels in the hindgut and spleen of turbots. The results indicate that the phage has a promising potential for therapeutic use against E. piscicida as the antimicrobial alternative to antibiotics in aquaculture.
多药耐药（MDR）细菌的传播对水产养殖业的发展构成了威胁。由于噬菌体具有有效的杀菌能力，因此被认为是抗生素的替代品，可以减少由 MDR 细菌引起的感染。在这项研究中，我们新分离并鉴定了两种爱德华氏菌噬菌体，以预防或治疗水产养殖中的感染。根据基因组序列和形态分析，这两种噬菌体分别被指定为属于肌尾病毒科和短尾病毒科的 vB_EpM_ZHS 和 vB_EpP_ZHX。vB_EpM_ZHS 和 vB_EpP_ZHX 的组合在体外和体内都提高了治疗效果。噬菌体鸡尾酒在体外显著抑制了细菌的生长，并降低了斑马鱼和大菱鲆感染爱德华氏菌后的死亡率约 40%，细菌负荷减少了一个数量级。此外，噬菌体鸡尾酒还增加了肿瘤坏死因子-α（TNF-α）、白细胞介素-12（IL-12）、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）的转录水平，并减轻了大菱鲆后肠和脾脏的炎症水平。这些结果表明，噬菌体作为水产养殖中抗生素的替代抗菌药物，具有治疗爱德华氏菌感染的巨大潜力。
