Nguyen Thi Xuan, Agazzi Alessandro, McGill Suzanne, Weidt Stefan, Han Quang Hanh, Gelemanović Andrea, McLaughlin Mark, Piñeiro Matilde, Savoini Giovanni, Eckersall Peter David, Burchmore Richard
Università degli Studi di Milano, Via dell'Università, 6, 26900 Lodi, Italy; University of Glasgow, Bearsden Rd, G61 1QH, United Kingdom; Vietnam National University of Agriculture, Hanoi, Viet Nam.
Università degli Studi di Milano, Via dell'Università, 6, 26900 Lodi, Italy.
J Proteomics. 2022 May 30;260:104562. doi: 10.1016/j.jprot.2022.104562. Epub 2022 Mar 18.
This study aimed to investigate the characteristic proteomic pattern of plasma from sows supplemented with low dietary ω6:ω3 fatty acids (FAs) ratio during gestation and lactation. Two dietary treatments (n = 8 each) comprised either a control ratio of ω6:ω3 FAs (CR, 13:1 during gestation and 10:1 during lactation) or a low ratio (LR, 4:1 during gestation and lactation) by adding soybean oil or linseed oil, respectively. High-resolution mass spectrometry-based quantitative proteomics was applied on plasma (n = 5 each) at day 108 of gestation (G108) and at the end of lactation (L-End), and a total of 379 proteins and 202 master proteins were identified. Out of these, four differentially abundant proteins between LR and CR samples at G108 may relate to serine-type endopeptidase inhibitor activity. Differentially abundant proteins in L-End versus G108 (12 up-regulated and 10 down-regulated) were positively correlated with the events that regulate plasma lipoproteins, stimulus- and defence-responses. These findings demonstrate the benefit of increased dietary ω3 FAs in modifying proteins involved in protective mechanisms against increased stresses in key life cycle phases in pigs. In addition, proteome changes from late gestation to late lactation disclosed the underlying mechanism of pigs in response to reproduction-related stimuli. SIGNIFICANCE: This study aimed to provide a proteomics insight into the beneficial effects of maternal diet supplementation with a low ω6:ω3 fatty acids ratio, based on previously reported performance and zootechnical data. The results suggest that a low dietary ω6:ω3 fatty acids ratio could enhance the cellular defence mechanisms against increased stresses and in particular to oxidative stress in sows during gestation and lactation, as reflected in proteomic changes of haptoglobin (HP), alpha-1-antitrypsin (SERPINA1) and serum amyloid P-component (APCS). Furthermore, significantly changed proteome profiles in sow plasma between late gestation and lactation phases have been revealed for the first time. This finding identified the adaptation mechanisms of sows to changing physiological events during reproduction.
本研究旨在探究在妊娠和哺乳期日粮中ω6:ω3脂肪酸（FAs）比例较低的母猪血浆的特征蛋白质组模式。两种日粮处理（每组n = 8）分别通过添加大豆油或亚麻籽油，构成ω6:ω3 FAs的对照比例（CR，妊娠期间为13:1，哺乳期间为10:1）或低比例（LR，妊娠和哺乳期间均为4:1）。基于高分辨率质谱的定量蛋白质组学应用于妊娠第108天（G108）和哺乳期末（L-End）的血浆样本（每组n = 5），共鉴定出379种蛋白质和202种主蛋白质。其中，在G108时LR和CR样本之间有四种差异丰富的蛋白质可能与丝氨酸型内肽酶抑制剂活性有关。L-End与G108相比差异丰富的蛋白质（12种上调和10种下调）与调节血浆脂蛋白、刺激和防御反应的事件呈正相关。这些发现证明了增加日粮ω3 FAs对调节参与猪关键生命周期阶段抗应激保护机制的蛋白质的益处。此外，从妊娠后期到哺乳后期的蛋白质组变化揭示了猪对繁殖相关刺激的潜在反应机制。意义：本研究旨在基于先前报道的生产性能和畜牧学数据，从蛋白质组学角度深入了解母体日粮补充低ω6:ω3脂肪酸比例的有益效果。结果表明，低日粮ω6:ω3脂肪酸比例可增强母猪在妊娠和哺乳期抵御应激增加，特别是氧化应激的细胞防御机制，这在触珠蛋白（HP）、α-1抗胰蛋白酶（SERPINA1）和血清淀粉样蛋白P成分（APCS）的蛋白质组变化中得到体现。此外，首次揭示了妊娠后期和哺乳期间母猪血浆中显著变化的蛋白质组图谱。这一发现确定了母猪在繁殖过程中对生理事件变化的适应机制。
