Università degli Studi di Milano, Via dell'Università, 6, 26900 Lodi, Italy; University of Glasgow, Bearsden Rd, G61 1QH, United Kingdom; Vietnam National University of Agriculture, Hanoi, Viet Nam.
Università degli Studi di Milano, Via dell'Università, 6, 26900 Lodi, Italy.
J Proteomics. 2023 Jan 6;270:104740. doi: 10.1016/j.jprot.2022.104740. Epub 2022 Sep 30.
This study examines whether maternal low ω6:ω3 ratio diet and offspring SW supplementation can improve offspring immunity and performance by elucidating the effects on piglet serum proteome. A total of 16 sows were given either a standard (CR, 13:1) or low ω6:ω3 ratio diet (LR, 4:1) during pregnancy and lactation and their male weaned piglets were supplemented with SW powder (4 g/kg, SW) or not (CT) in a 21-day post-weaning (PW) diet. Four PW piglet groups were then identified based on dam and piglet treatment, namely CRCT, CRSW, LRCT, and LRSW (n = 10 each). Piglet serum collected at weaning and d21 PW were analysed (n = 5 each) using TMT-based quantitative proteomics and validated by appropriate assays. The differentially abundant proteins (n = 122) displayed positive effects of maternal LR diet on anti-inflammatory properties and innate immune stimulation. Progeny SW diet activated the innate immunity and enhance the host defence during inflammation. These data demonstrate the value of decreasing ω6:ω3 ratio in maternal diet and SW supplementation in PW piglet's diet to boost their immunity and anti-inflammation properties. SIGNIFICANCE: This novel proteomic study in post-weaned piglets addresses the interplay between maternal and offspring nutritional interventions in a context of rapid and dynamic alterations in piglet metabolic status around weaning. Decreasing ω6:ω3 ratio in maternal diet and SW supplementation in PW piglet's diet can boost their immunity and anti-inflammation properties. This study also provides new insights into piglet serum proteome regulation during post-weaning, a critical development period in swine.
本研究通过阐明对仔猪血清蛋白质组的影响，研究了母体低 ω6:ω3 比例饮食和后代 SW 补充是否可以改善后代的免疫力和性能。在妊娠和哺乳期，16 头母猪分别给予标准（CR，13:1）或低 ω6:ω3 比例饮食（LR，4:1），其断奶仔猪在断奶后 21 天（PW）饮食中补充 SW 粉（4 g/kg，SW）或不补充（CT）。然后根据母鼠和幼鼠的处理，将 4 个 PW 仔猪组分为 CRCT、CRSW、LRCT 和 LRSW（每组 10 只）。在断奶时和 PW d21 收集仔猪血清进行分析（每组 5 只），使用 TMT 定量蛋白质组学进行分析，并通过适当的测定进行验证。差异丰度蛋白（n=122）显示母体 LR 饮食对抗炎特性和先天免疫刺激具有积极影响。后代 SW 饮食激活了先天免疫，并在炎症期间增强了宿主防御。这些数据表明，降低母体饮食中的 ω6:ω3 比例和在 PW 仔猪饮食中补充 SW 以提高其免疫力和抗炎特性具有重要价值。意义：本研究通过对断奶仔猪进行蛋白质组学研究，在仔猪代谢状态快速和动态变化的背景下，探讨了母体和后代营养干预之间的相互作用。降低母体饮食中的 ω6:ω3 比例和在 PW 仔猪饮食中补充 SW 可以提高其免疫力和抗炎特性。本研究还为断奶后仔猪血清蛋白质组的调节提供了新的见解，这是猪的一个关键发育阶段。
