新种和新种，两种来自人工湿地的细菌。
sp. nov. and sp. nov., two bacteria from constructed wetland.
机构信息
State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
State Key Laboratory of Microbial Resources and Environmental Microbiology Research Center at Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
出版信息
Int J Syst Evol Microbiol. 2022 Mar;72(3). doi: 10.1099/ijsem.0.005280.
Two Gram-stain-negative, aerobic, non-motile and rod-shaped bacterial strains designated 3A5MI-3 and RSS-23 were isolated from the Dragon-shaped Wetland System in Beijing Olympic Park, PR China. Strain 3A5MI-3 grew at 15–45 °C, pH 5.0–9.0 and with 0–2 % NaCl (w/v), and strain RSS-23 grew at 15-40 C, pH 5.5–9.0 and with 0–1 % NaCl (w/v). Phylogenetic analyses of 16S rRNA gene sequences revealed that strains 3A5MI-3 and RSS-23 were members of and , respectively. Phylogenetically closest relatives of strains 3A5MI-3 and RSS-23 were R384 and SY21, respectively. The cells of strain 3A5MI-3 contained menaquinone MK-7 and phosphatidylethanolamine, and the major cellular fatty acids were composed of iso-C, iso-C ω6 and/or iso-C ω7, iso-C 3-OH, C and summed feature 3 (C ω7/C ω6). Strain RSS-23 contained ubiquinone Q-8 and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unknown phospholipids and an unknown lipid, and its major cellular fatty acids were iso-C, iso-C ω9, iso-C 3-OH and summed feature 3 (C ω7/C ω6). DNA sequencing resulted in 6.59 Mb for the strain 3A5MI-3 genome and 2.79 Mb for the strain RSS-23 genome. The calculated G+C molar contents for strains 3A5MI-3 and RSS-23 were 47.07 and 61.21 mol%, respectively. According to phenotypic and phylogenetic characteristics, strains 3A5MI-3 and RSS-23 represent novel species of the genera and for which the names sp. nov. and sp. nov. are proposed. The type strain for sp. nov. is 3A5MI-3 (=CGMCC 1.17737=KCTC 82817). The type strain for sp. nov. is RSS-23 (=CGMCC 1.17738=KCTC 82820).
两株革兰氏阴性、需氧、不运动的杆状细菌菌株，分别命名为 3A5MI-3 和 RSS-23，从中国北京奥林匹克公园的龙形湿地系统中分离得到。菌株 3A5MI-3 在 15-45°C、pH5.0-9.0 和 0-2%NaCl(w/v)下生长，而 RSS-23 在 15-40°C、pH5.5-9.0 和 0-1%NaCl(w/v)下生长。16S rRNA 基因序列的系统发育分析表明，菌株 3A5MI-3 和 RSS-23 分别属于 和 。菌株 3A5MI-3 和 RSS-23 的最接近的亲缘关系分别是 R384 和 SY21。菌株 3A5MI-3 的细胞含有menaquinone MK-7 和磷脂酰乙醇胺，主要的细胞脂肪酸由 iso-C、iso-Cω6 和/或 iso-Cω7、iso-C 3-OH、C 和 sum feature 3 (Cω7/Cω6)组成。菌株 RSS-23 含有 ubiquinone Q-8 和双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、两种未知的磷脂和一种未知的脂质，其主要的细胞脂肪酸是 iso-C、iso-Cω9、iso-C 3-OH 和 sum feature 3 (Cω7/Cω6)。DNA 测序结果显示，菌株 3A5MI-3 的基因组大小为 6.59 Mb，菌株 RSS-23 的基因组大小为 2.79 Mb。菌株 3A5MI-3 和 RSS-23 的计算 G+C 摩尔含量分别为 47.07%和 61.21%。根据表型和系统发育特征，菌株 3A5MI-3 和 RSS-23 代表了 和 属的新种，分别命名为 sp. nov.和 sp. nov.。新种的模式菌株为 3A5MI-3(=CGMCC 1.17737=KCTC 82817)。新种的模式菌株为 RSS-23(=CGMCC 1.17738=KCTC 82820)。
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