Dahal Ram Hari, Kim Jaisoo
Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Kyonggi-Do 443-760, South Korea.
Int J Syst Evol Microbiol. 2016 Jul;66(7):2650-2656. doi: 10.1099/ijsem.0.001099. Epub 2016 Apr 20.
An aerobic, Gram-stain-negative, oxidase-positive and catalase-negative, non-motile, non-spore-forming, rod-shaped, orange-pigmented bacterium designated strain R384 was isolated from soil. Flexirubin-type pigments were present. Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain R384 formed a lineage within the family Chitinophagaceae of the phylum Bacteroidetes that was distinct from various species of the genus Niabella, including 'Niabella thaonhiensis' NHI-24 (98.47 % sequence similarity), Niabellaaurantiaca DSM 17617 (96.89 %), Niabelladrilacis E90 (96.63 %), Niabellahirudinis E96 (96.61 %), Niabellatibetensis 15-4 (95.53 %), Niabellasoli DSM 19437 (94.81 %), Niabellaginsengisoli GR10-1 (94.19 %) and Niabellayanshanensis CCBAU 05354 (93.67 %). The major isoprenoid quinone was menaquinone-7 (MK-7) and the major polar lipid was phosphatidylethanolamine. The major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH. The DNA G+C content of strain R384was 44.7 mol %. DNA-DNA hybridization values between strain R384 and other members of the genus Niabella ranged from 26 to 55 %. On the basis of phenotypic, genotypic and phylogenetic analysis, strain R384 represents a novel species of the genus Niabella, for which the name Niabellapedocola sp. nov. is proposed. The type strain is R384 (=KEMB 9005-329=KACC 18454=JCM 31011).
从土壤中分离出一株需氧、革兰氏染色阴性、氧化酶阳性、过氧化氢酶阴性、无运动性、不形成芽孢、杆状、产橙色色素的细菌，命名为菌株R384。存在柔韧菌素型色素。基于其16S rRNA基因序列的系统发育分析表明，菌株R384在拟杆菌门噬几丁质菌科内形成了一个分支，该分支与Niabella属的各种物种不同，包括“泰国Niabella”NHI-24（序列相似性98.47%）、橙色Niabella DSM 17617（96.89%）、钻孔Niabella E90（96.63%）、水蛭Niabella E96（96.61%）、西藏Niabella 15-4（95.53%）、土壤Niabella DSM 19437（94.81%）、人参土Niabella GR10-1（94.19%）和燕山Niabella CCBAU 05354（93.67%）。主要类异戊二烯醌为甲基萘醌-7（MK-7），主要极性脂为磷脂酰乙醇胺。主要细胞脂肪酸为异C15 : 0、异C15 : 1 G、总和特征3（C16 : 1ω7c/C16 : 1ω6c）和异C17 : 0 3-OH。菌株R384的DNA G+C含量为44.7 mol%。菌株R384与Niabella属其他成员之间的DNA-DNA杂交值在26%至55%之间。基于表型、基因型和系统发育分析，菌株R384代表Niabella属的一个新物种，为此提出新名称土壤Niabella（Niabellapedocola sp. nov.）。模式菌株为R384（=KEMB 9005-329=KACC 18454=JCM 31011）。
