Warrier R, Jayaraman R
Mol Gen Genet. 1986 Sep;204(3):463-8. doi: 10.1007/BF00331025.
The incompatibility between F plasmids is transiently suppressed in Escherichia coli strain CSH54. As a result this strain is able to maintain two F' factors or an F' factor and a mini-F plasmid for considerably longer periods than normal strains. When selective pressure for two markers carried by two separate F's (or an F' and mini-F) is imposed on normal strains, the two plasmids tend to form a cointegrate structure which can be detected genetically by the joint transfer of both the markers upon mating. This does not happen in CSH54; instead, the two plasmids are maintained and transferred independently. Physical evidence for the maintenance of an F' and a mini-F plasmid is provided by agarose gel electrophoresis.
在大肠杆菌菌株CSH54中，F质粒之间的不相容性被短暂抑制。因此，与正常菌株相比，该菌株能够在相当长的时间内维持两个F'因子或一个F'因子和一个微型F质粒。当对由两个单独的F's（或一个F'和微型F）携带的两个标记施加选择压力时，正常菌株中的两个质粒倾向于形成共整合结构，通过交配时两个标记的联合转移可以从遗传学上检测到这种结构。而在CSH54中不会发生这种情况；相反，这两个质粒会独立维持和转移。琼脂糖凝胶电泳为维持一个F'和一个微型F质粒提供了物理证据。
