Chair for Biomarkers of Chronic Diseases, Biochemistry Department, College of Science, King Saud University, PO Box, 2455, Riyadh, 11451, Saudi Arabia.
Sci Rep. 2022 Mar 22;12(1):4908. doi: 10.1038/s41598-022-09022-1.
Epidemiological studies suggest that the Zinc-α-2-glycoprotein (ZAG) plays significant physiological roles. In this study we investigate whether ZAG could be considered as a clinical biomarker in the diagnosis and prognosis of metabolic syndrome (MetS) in Saudi population. As such insights urgently required for management of MetS. Thus, we have determined serum levels of ZAG in patients with MetS and normal individuals. We have also assessed the correlation between ZAG and different components of MetS. In this case-control study, clinical information of 200 Saudi male and female subjects (age range 30-65) with MetS (n = 100) and healthy controls (n = 100) were extracted from the database of the Chair of Biomarkers of Chronic Disease (CBCD) in King Saud University (KSU), Riyadh, Saudi Arabia. MetS was screened according to NCEP ATP III criteria (National Cholesterol Education Program Adult Treatment Panel III). Fasting glucose and lipid profile levels were measured using Konelab. Serum TNF-α, IL- 6, CRP and ZAG levels were measured using commercially available assays. There was an age-dependent significant increase in ZAG level among MetS subjects than controls (43.8 ± 19.5 vs 48.1 ± 14.8; P = 0.04). A significant inverse correlation between ZAG and serum HDL-cholesterol (r = - 0.20, P < 0.05) was observed. Whereas, triglycerides (r = 0.25, P < 0.01), waist circumference (WHR) (r = 0.17, P < 0.05) and CRP (r = 0.24, P < 0.01) were all significantly and positively associated with ZAG. Circulating ZAG is associated with MetS in an age-dependent manner. Serum ZAG is a potential biomarker for MetS.
流行病学研究表明，锌-α-2-糖蛋白（ZAG）在生理上起着重要作用。在这项研究中，我们研究了 ZAG 是否可以被认为是沙特人群代谢综合征（MetS）诊断和预后的临床生物标志物。因此，我们需要深入了解 MetS 的管理。因此，我们测定了 MetS 患者和正常人的血清 ZAG 水平。我们还评估了 ZAG 与 MetS 不同成分之间的相关性。在这项病例对照研究中，从沙特阿拉伯利雅得沙特国王大学（KSU）慢性疾病生物标志物主席（CBCD）的数据库中提取了 200 名沙特男性和女性受试者（年龄范围 30-65 岁）的临床信息，其中包括 MetS（n=100）和健康对照组（n=100）。MetS 根据 NCEP ATP III 标准（国家胆固醇教育计划成人治疗专家组 III）进行筛查。空腹血糖和血脂水平使用 Konelab 进行测量。使用商业试剂盒测量血清 TNF-α、IL-6、CRP 和 ZAG 水平。与对照组相比，MetS 患者的 ZAG 水平随年龄呈显著增加（43.8±19.5 vs 48.1±14.8；P=0.04）。观察到 ZAG 与血清高密度脂蛋白胆固醇（r=-0.20，P<0.05）呈显著负相关。然而，甘油三酯（r=0.25，P<0.01）、腰围（WHR）（r=0.17，P<0.05）和 CRP（r=0.24，P<0.01）均与 ZAG 呈显著正相关。循环 ZAG 与 MetS 呈年龄依赖性相关。血清 ZAG 是 MetS 的潜在生物标志物。
