Obaseiki-Ebor E E, Obasi E E
Mutat Res. 1986 Oct;175(2):51-9. doi: 10.1016/0165-7992(86)90125-9.
Using the Ames plate reversion and fluctuation tests, the mutagenic activity of chloroquine was tested in the new tester strains of Salmonella typhimurium, TA97, TA102, and Escherichia coli strains WP2, WP2hcr, WP6 and WP67. The E. coli transconjugants obtained from the mating transfer of R-plasmid(s) in strains TA97 and TA102 respectively to E. coli WP2, i.e. EE97 and EE102, were also tested. Chloroquine reverted strain TA97 from histidine dependence to independence and also reverted E. coli strains EE97 and EE102 from tryptophan dependence to independence. The E. coli strains WP2, WP2hcr; WP6 and WP67 and S. typhimurium TA102 were not affected. S. typhimurium TA97 could be reverted with 250 ng/ml of chloroquine (therapeutic blood level of chloroquine is 300 ng/ml). Reversion generally occurred optimally at the relatively lower concentrations of chloroquine i.e. 25, 50 micrograms/ml than at higher concentrations. From the properties of the reverted tester strains, the results indicated that chloroquine per se mediated frameshift reversion.
利用艾姆斯平板回复突变试验和波动试验，在鼠伤寒沙门氏菌新测试菌株TA97、TA102以及大肠杆菌菌株WP2、WP2hcr、WP6和WP67中检测了氯喹的诱变活性。还对分别从TA97和TA102菌株中的R质粒与大肠杆菌WP2的接合转移获得的大肠杆菌转接合子（即EE97和EE102）进行了检测。氯喹使TA97菌株从组氨酸依赖回复为不依赖，也使大肠杆菌菌株EE97和EE102从色氨酸依赖回复为不依赖。大肠杆菌菌株WP2、WP2hcr、WP6和WP67以及鼠伤寒沙门氏菌TA102未受影响。250 ng/ml的氯喹可使鼠伤寒沙门氏菌TA97回复突变（氯喹的治疗血药浓度为300 ng/ml）。回复突变通常在氯喹相对较低的浓度（即25、50微克/毫升）下比在较高浓度下更易发生。从回复突变测试菌株的特性来看，结果表明氯喹本身介导了移码回复突变。
