Departamento de Tecnologia e Inspeção de Produtos de Origem Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Ecohealth. 2022 Mar;19(1):75-84. doi: 10.1007/s10393-022-01583-x. Epub 2022 Mar 22.
Dengue virus (DENV) 1-4 is the etiological agent of dengue, the most important viral infection transmitted by Aedes spp mosquitoes to humans. Our goal was to identify the circulating DENV in Aedes aegypti collected in an area of Brazil where all four DENV serotypes had already been detected in humans, understand the epidemiology better, and to test the vector as a virological surveillance tool. Twenty-eight larvae pools and 174 females of Aedes aegypti were screened by reverse transcriptase quantitative polymerase chain reaction and semi-nested PCR assays. PCR products were sequenced, and phylogenetic analyses were performed. Nine larvae pools (32.1%) were positive for DENV, four (44.4%) with DENV-3, and five (55.6%) with more than one serotype. Fifteen females (8.6%) were positive for any DENV serotype. DENV-1 isolates belong to genotype V, DENV-2 to American-Asian genotype, DENV-3 to genotypes I and III, and DENV-4 to genotypes I and II. We demonstrate for the first time the co-circulation of all four DENV serotypes in larvae pools and adult Aedes aegypti in a hyperendemic area. This scenario represents a challenge for disease control and reinforces the importance of virological surveillance in the vector as a tool for predicting circulating DENV serotypes in humans.
登革病毒（DENV）1-4 是登革热的病原体，是由埃及伊蚊传播给人类的最重要的病毒感染。我们的目标是鉴定在巴西一个地区采集的埃及伊蚊中循环的登革热病毒，该地区已在人类中检测到所有四种登革热病毒血清型，以更好地了解流行病学情况，并测试该媒介作为病毒学监测工具。通过逆转录定量聚合酶链反应和半巢式 PCR 检测了 28 个幼虫池和 174 只埃及伊蚊雌蚊。对 PCR 产物进行测序，并进行系统发育分析。9 个幼虫池（32.1%）对登革热病毒呈阳性，其中 4 个（44.4%）为 DENV-3，5 个（55.6%）为多种血清型。15 只雌蚊（8.6%）对任何一种登革热病毒血清型呈阳性。DENV-1 分离株属于基因型 V，DENV-2 属于美洲-亚洲基因型，DENV-3 属于基因型 I 和 III，DENV-4 属于基因型 I 和 II。我们首次证明在一个高度流行地区的幼虫池和成年埃及伊蚊中同时存在所有四种登革热病毒血清型。这种情况对疾病控制构成了挑战，并加强了病毒学监测在媒介中的重要性，可作为预测人类循环登革热病毒血清型的工具。
