登革病毒在亚马逊流域埃及伊蚊中的经卵传递：一种异种监测的本地模型
Transovarial transmission of DENV in Aedes aegypti in the Amazon basin: a local model of xenomonitoring.
作者信息
da Costa Cristiano Fernandes, Dos Passos Ricardo Augusto, Lima José Bento Pereira, Roque Rosemary Aparecida, de Souza Sampaio Vanderson, Campolina Thais Bonifácio, Secundino Nágila Francinete Costa, Pimenta Paulo Filemon Paolucci
机构信息
Department of Environmental Surveillance, Health Surveillance Foundation of Amazonas State FVS-AM, Av. Torquato Tapajós, 6132, Colônia Santo Antônio, Zip 69.093-018, Manaus, Amazonas, Brazil.
Universidade Nilton Lins, Programa de Pró Reitoria de Pesquisa e Pós-Graduação - UNICENTER. Laboratório de Entomologia Aplicada, Office 160, Av. Professor Nilton Lins 3259, Parque das Laranjeiras, Zip: 69 058-030, Manaus, Amazonas, Brazil.
出版信息
Parasit Vectors. 2017 May 19;10(1):249. doi: 10.1186/s13071-017-2194-5.
BACKGROUND
Transovarial transmission of dengue virus in Aedes spp. mosquitoes is considered an important mechanism for the maintenance of the virus in nature and may be implicated in the occurrence of outbreaks and epidemics of the disease. However, there are few studies involving transovarial transmission and viral vector monitoring as a surveillance tool and control strategy. The present study evaluated transovarial transmission of dengue virus in Aedes aegypti populations as a xenomonitoring strategy in municipalities of the Amazonas state.
RESULTS
Aedes sp. eggs (13.164) were collected, with 30% viability of third- and fourth-instar larvae. Transovarial transmission of DENV was detected in all municipalities. The transovarial infection rate (TOR) in the municipalities was 46% of the DENV positive samples. The minimum infection rate (MIR) was 17.7 in the state, varying from 11.4 to 24.1 per 1,000 larvae tested in the respective municipalities. Four DENV serotypes were identified, with DENV I and IV being present in all municipalities investigated. The number of reported dengue fever cases varied during this period.
CONCLUSIONS
Our results suggest that transovarial transmission may be an important mechanism for the maintenance and spreading of the disease in Amazonas municipalities. Using qRT-PCR, it was possible to identify the four DENV serotypes in larval samples. The methodology used in the present study proved suitable as a DENV xenomonitoring model in immature mosquitoes, contributing to the development of systems for early detection of viral circulation and predictive models for the occurrence of outbreaks and epidemics of this disease.
TRIAL REGISTRATION
CAAE34025414200005015 .
背景
登革病毒在伊蚊属蚊子中的经卵传播被认为是该病毒在自然界中维持的重要机制，并且可能与疾病的暴发和流行有关。然而，很少有研究将经卵传播和病毒载体监测作为一种监测工具和控制策略。本研究评估了亚马逊州各城市埃及伊蚊种群中登革病毒的经卵传播情况，作为一种外来监测策略。
结果
收集了伊蚊属卵（13164枚），三龄和四龄幼虫的存活率为30%。在所有城市均检测到登革病毒的经卵传播。各城市的经卵感染率（TOR）为登革病毒阳性样本的46%。该州的最小感染率（MIR）为17.7，各城市每1000只检测幼虫中的感染率在11.4至24.1之间。鉴定出四种登革病毒血清型，登革病毒I型和IV型在所有调查城市中均有出现。在此期间报告的登革热病例数有所变化。
结论
我们的结果表明，经卵传播可能是亚马逊州各城市中该疾病维持和传播的重要机制。使用定量逆转录聚合酶链反应（qRT-PCR），可以在幼虫样本中鉴定出四种登革病毒血清型。本研究中使用的方法被证明适用于未成熟蚊子中的登革病毒外来监测模型，有助于开发病毒传播早期检测系统以及该疾病暴发和流行的预测模型。
试验注册号
CAAE34025414200005015 。
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